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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella putrefaciens Putative pterin-4-alpha-carbinolamine dehydratase (Sputcn32_1387) CSB-YP396564STQ
CSB-EP396564STQ
CSB-BP396564STQ
CSB-MP396564STQ
CSB-EP396564STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Protein TolB (tolB) CSB-YP396565STQ
CSB-EP396565STQ
CSB-BP396565STQ
CSB-MP396565STQ
CSB-EP396565STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Na (+)/H (+) antiporter nhaB, partial CSB-YP396566STQ
CSB-EP396566STQ
CSB-BP396566STQ
CSB-MP396566STQ
CSB-EP396566STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens tRNA (mo5U34)-methyltransferase CSB-YP396567STQ
CSB-EP396567STQ
CSB-BP396567STQ
CSB-MP396567STQ
CSB-EP396567STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Serine--tRNA ligase (serS) CSB-YP396568STQ
CSB-EP396568STQ
CSB-BP396568STQ
CSB-MP396568STQ
CSB-EP396568STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 50S ribosomal protein L20 (rplT) CSB-YP396569STQ
CSB-EP396569STQ
CSB-BP396569STQ
CSB-MP396569STQ
CSB-EP396569STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Co-chaperone protein hscB homolog (hscB) CSB-YP396570STQ
CSB-EP396570STQ
CSB-BP396570STQ
CSB-MP396570STQ
CSB-EP396570STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP396571STQ
CSB-EP396571STQ
CSB-BP396571STQ
CSB-MP396571STQ
CSB-EP396571STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Nucleoid-associated protein Sputcn32_2288 (Sputcn32_2288) CSB-YP396572STQ
CSB-EP396572STQ
CSB-BP396572STQ
CSB-MP396572STQ
CSB-EP396572STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Tryptophan synthase beta chain (trpB) CSB-YP396573STQ
CSB-EP396573STQ
CSB-BP396573STQ
CSB-MP396573STQ
CSB-EP396573STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP396574STQ
CSB-EP396574STQ
CSB-BP396574STQ
CSB-MP396574STQ
CSB-EP396574STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0312 protein Sputcn32_2702 (Sputcn32_2702) CSB-YP396575STQ
CSB-EP396575STQ
CSB-BP396575STQ
CSB-MP396575STQ
CSB-EP396575STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP396576STQ
CSB-EP396576STQ
CSB-BP396576STQ
CSB-MP396576STQ
CSB-EP396576STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP396577STQ
CSB-EP396577STQ
CSB-BP396577STQ
CSB-MP396577STQ
CSB-EP396577STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Peptidyl-tRNA hydrolase (pth) CSB-YP396578STQ
CSB-EP396578STQ
CSB-BP396578STQ
CSB-MP396578STQ
CSB-EP396578STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP396579STQ
CSB-EP396579STQ
CSB-BP396579STQ
CSB-MP396579STQ
CSB-EP396579STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Cell volume regulation protein A homolog (cvrA), partial CSB-YP396580STQ
CSB-EP396580STQ
CSB-BP396580STQ
CSB-MP396580STQ
CSB-EP396580STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens ATP-dependent RNA helicase rhlB (rhlB) CSB-YP396581STQ
CSB-EP396581STQ
CSB-BP396581STQ
CSB-MP396581STQ
CSB-EP396581STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP396582STQ
CSB-EP396582STQ
CSB-BP396582STQ
CSB-MP396582STQ
CSB-EP396582STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Protein CyaY (cyaY) CSB-YP396583STQ
CSB-EP396583STQ
CSB-BP396583STQ
CSB-MP396583STQ
CSB-EP396583STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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