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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas mendocina Membrane protein insertase YidC (yidC), partial CSB-YP396965PZM1
CSB-EP396965PZM1
CSB-BP396965PZM1
CSB-MP396965PZM1
CSB-EP396965PZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Chromosomal replication initiator protein DnaA (dnaA) CSB-YP396966STQ
CSB-EP396966STQ
CSB-BP396966STQ
CSB-MP396966STQ
CSB-EP396966STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Phosphate propanoyltransferase (pduL) CSB-YP396967STQ
CSB-EP396967STQ
CSB-BP396967STQ
CSB-MP396967STQ
CSB-EP396967STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP396968STQ
CSB-EP396968STQ
CSB-BP396968STQ
CSB-MP396968STQ
CSB-EP396968STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0758 protein Sputcn32_0462 (Sputcn32_0462) CSB-YP396969STQ
CSB-EP396969STQ
CSB-BP396969STQ
CSB-MP396969STQ
CSB-EP396969STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP396970STQ
CSB-EP396970STQ
CSB-BP396970STQ
CSB-MP396970STQ
CSB-EP396970STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Sec-independent protein translocase protein TatB (tatB), partial CSB-YP396971STQ
CSB-EP396971STQ
CSB-BP396971STQ
CSB-MP396971STQ
CSB-EP396971STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP396972STQ
CSB-EP396972STQ
CSB-BP396972STQ
CSB-MP396972STQ
CSB-EP396972STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0231 protein Sputcn32_0682 (Sputcn32_0682) CSB-YP396973STQ
CSB-EP396973STQ
CSB-BP396973STQ
CSB-MP396973STQ
CSB-EP396973STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 30S ribosomal protein S9 (rpsI) CSB-YP396974STQ
CSB-EP396974STQ
CSB-BP396974STQ
CSB-MP396974STQ
CSB-EP396974STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Alanine racemase (alr) CSB-YP396975STQ
CSB-EP396975STQ
CSB-BP396975STQ
CSB-MP396975STQ
CSB-EP396975STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP396976STQ
CSB-EP396976STQ
CSB-BP396976STQ
CSB-MP396976STQ
CSB-EP396976STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP396977STQ
CSB-EP396977STQ
CSB-BP396977STQ
CSB-MP396977STQ
CSB-EP396977STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens DNA mismatch repair protein mutH (mutH) CSB-YP396978STQ
CSB-EP396978STQ
CSB-BP396978STQ
CSB-MP396978STQ
CSB-EP396978STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 50S ribosomal protein L19 (rplS) CSB-YP396979STQ
CSB-EP396979STQ
CSB-BP396979STQ
CSB-MP396979STQ
CSB-EP396979STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens SsrA-binding protein (smpB) CSB-YP396980STQ
CSB-EP396980STQ
CSB-BP396980STQ
CSB-MP396980STQ
CSB-EP396980STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP396981STQ
CSB-EP396981STQ
CSB-BP396981STQ
CSB-MP396981STQ
CSB-EP396981STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Putative reductase Sputcn32_1495 (Sputcn32_1495) CSB-YP396982STQ
CSB-EP396982STQ
CSB-BP396982STQ
CSB-MP396982STQ
CSB-EP396982STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Uracil phosphoribosyltransferase (upp) CSB-YP396983STQ
CSB-EP396983STQ
CSB-BP396983STQ
CSB-MP396983STQ
CSB-EP396983STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0502 protein Sputcn32_1644 (Sputcn32_1644) CSB-YP396984STQ
CSB-EP396984STQ
CSB-BP396984STQ
CSB-MP396984STQ
CSB-EP396984STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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