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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Metallosphaera sedula Glucose 1-dehydrogenase (gdh) CSB-YP397025MNM
CSB-EP397025MNM
CSB-BP397025MNM
CSB-MP397025MNM
CSB-EP397025MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 50S ribosomal protein L11P (rpl11p) CSB-YP397026MNM
CSB-EP397026MNM
CSB-BP397026MNM
CSB-MP397026MNM
CSB-EP397026MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Anthranilate phosphoribosyltransferase (trpD) CSB-YP397027MNM
CSB-EP397027MNM
CSB-BP397027MNM
CSB-MP397027MNM
CSB-EP397027MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula tRNA (guanine (26)-N (2))-dimethyltransferase CSB-YP397028MNM
CSB-EP397028MNM
CSB-BP397028MNM
CSB-MP397028MNM
CSB-EP397028MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Riboflavin kinase (ribK) CSB-YP397029MNM
CSB-EP397029MNM
CSB-BP397029MNM
CSB-MP397029MNM
CSB-EP397029MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Chromatin protein Cren7 (creN7) CSB-YP397030MNM
CSB-EP397030MNM
CSB-BP397030MNM
CSB-MP397030MNM
CSB-EP397030MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Chorismate synthase (aroC) CSB-YP397031MNM
CSB-EP397031MNM
CSB-BP397031MNM
CSB-MP397031MNM
CSB-EP397031MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Ketol-acid reductoisomerase (ilvC) CSB-YP397032MNM
CSB-EP397032MNM
CSB-BP397032MNM
CSB-MP397032MNM
CSB-EP397032MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP397033MNM
CSB-EP397033MNM
CSB-BP397033MNM
CSB-MP397033MNM
CSB-EP397033MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula DNA-directed RNA polymerase subunit N (rpoN) CSB-YP397034MNM
CSB-EP397034MNM
CSB-BP397034MNM
CSB-MP397034MNM
CSB-EP397034MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Putative ribose 1,5-bisphosphate isomerase (Msed_2221) CSB-YP397035MNM
CSB-EP397035MNM
CSB-BP397035MNM
CSB-MP397035MNM
CSB-EP397035MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 30S ribosomal protein S6e (rps6e) CSB-YP397036MNM
CSB-EP397036MNM
CSB-BP397036MNM
CSB-MP397036MNM
CSB-EP397036MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Histidine--tRNA ligase (hisS) CSB-YP397037MNM
CSB-EP397037MNM
CSB-BP397037MNM
CSB-MP397037MNM
CSB-EP397037MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosome-binding factor A (rbfA) CSB-YP397038BPF
CSB-EP397038BPF
CSB-BP397038BPF
CSB-MP397038BPF
CSB-EP397038BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP397039BPF
CSB-EP397039BPF
CSB-BP397039BPF
CSB-MP397039BPF
CSB-EP397039BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP397040BPF
CSB-EP397040BPF
CSB-BP397040BPF
CSB-MP397040BPF
CSB-EP397040BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP397041BPF
CSB-EP397041BPF
CSB-BP397041BPF
CSB-MP397041BPF
CSB-EP397041BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP397042BPF
CSB-EP397042BPF
CSB-BP397042BPF
CSB-MP397042BPF
CSB-EP397042BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Putative pterin-4-alpha-carbinolamine dehydratase (BRADO0254) CSB-YP397043BPF
CSB-EP397043BPF
CSB-BP397043BPF
CSB-MP397043BPF
CSB-EP397043BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L19 (rplS) CSB-YP397044BPF
CSB-EP397044BPF
CSB-BP397044BPF
CSB-MP397044BPF
CSB-EP397044BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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