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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clavibacter michiganensis subsp. michiganensis Hydroxyethylthiazole kinase (thiM) CSB-YP396905CWT
CSB-EP396905CWT
CSB-BP396905CWT
CSB-MP396905CWT
CSB-EP396905CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP396906CWT
CSB-EP396906CWT
CSB-BP396906CWT
CSB-MP396906CWT
CSB-EP396906CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Cysteine--tRNA ligase (cysS) CSB-YP396907CWT
CSB-EP396907CWT
CSB-BP396907CWT
CSB-MP396907CWT
CSB-EP396907CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L17 (rplQ) CSB-YP396908CWT
CSB-EP396908CWT
CSB-BP396908CWT
CSB-MP396908CWT
CSB-EP396908CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L6 (rplF) CSB-YP396909CWT
CSB-EP396909CWT
CSB-BP396909CWT
CSB-MP396909CWT
CSB-EP396909CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L16 (rplP) CSB-YP396910CWT
CSB-EP396910CWT
CSB-BP396910CWT
CSB-MP396910CWT
CSB-EP396910CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 30S ribosomal protein S10 (rpsJ) CSB-YP396911CWT
CSB-EP396911CWT
CSB-BP396911CWT
CSB-MP396911CWT
CSB-EP396911CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L1 (rplA) CSB-YP396912CWT
CSB-EP396912CWT
CSB-BP396912CWT
CSB-MP396912CWT
CSB-EP396912CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 30S ribosomal protein S14 (rpsN) CSB-YP396913CWT
CSB-EP396913CWT
CSB-BP396913CWT
CSB-MP396913CWT
CSB-EP396913CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Putative membrane protein insertion efficiency factor (CMM_2976) CSB-YP396914CWT
CSB-EP396914CWT
CSB-BP396914CWT
CSB-MP396914CWT
CSB-EP396914CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP396915VDY
CSB-EP396915VDY
CSB-BP396915VDY
CSB-MP396915VDY
CSB-EP396915VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP396916PZM
CSB-EP396916PZM
CSB-BP396916PZM
CSB-MP396916PZM
CSB-EP396916PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Ribosomal protein S6 modification protein (rimK) CSB-YP396917PZM
CSB-EP396917PZM
CSB-BP396917PZM
CSB-MP396917PZM
CSB-EP396917PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Phosphoglycerate kinase (pgk) CSB-YP396918PZM
CSB-EP396918PZM
CSB-BP396918PZM
CSB-MP396918PZM
CSB-EP396918PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP396919PZM
CSB-EP396919PZM
CSB-BP396919PZM
CSB-MP396919PZM
CSB-EP396919PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 3-dehydroquinate synthase (aroB) CSB-YP396920PZM
CSB-EP396920PZM
CSB-BP396920PZM
CSB-MP396920PZM
CSB-EP396920PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 50S ribosomal protein L9 (rplI) CSB-YP396921PZM
CSB-EP396921PZM
CSB-BP396921PZM
CSB-MP396921PZM
CSB-EP396921PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Urease accessory protein UreF (ureF) CSB-YP396922PZM
CSB-EP396922PZM
CSB-BP396922PZM
CSB-MP396922PZM
CSB-EP396922PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Ribosomal protein L11 methyltransferase (prmA) CSB-YP396923PZM
CSB-EP396923PZM
CSB-BP396923PZM
CSB-MP396923PZM
CSB-EP396923PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP396924PZM
CSB-EP396924PZM
CSB-BP396924PZM
CSB-MP396924PZM
CSB-EP396924PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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