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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Meyerozyma guilliermondii UPF0507 protein PGUG_02975 (PGUG_02975), partial CSB-YP398226MTM
CSB-EP398226MTM
CSB-BP398226MTM
CSB-MP398226MTM
CSB-EP398226MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent RNA helicase HAS1 (HAS1), partial CSB-YP398227MTM
CSB-EP398227MTM
CSB-BP398227MTM
CSB-MP398227MTM
CSB-EP398227MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Autophagy-related protein 6 (ATG6) CSB-YP398228MTM
CSB-EP398228MTM
CSB-BP398228MTM
CSB-MP398228MTM
CSB-EP398228MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent RNA helicase DBP6 (DBP6), partial CSB-YP398229MTM
CSB-EP398229MTM
CSB-BP398229MTM
CSB-MP398229MTM
CSB-EP398229MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Cytosolic Fe-S cluster assembly factor NAR1 (NAR1), partial CSB-YP398230MTM
CSB-EP398230MTM
CSB-BP398230MTM
CSB-MP398230MTM
CSB-EP398230MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Altered inheritance of mitochondria protein 41, mitochondrial (AIM41) CSB-YP398231MTM
CSB-EP398231MTM
CSB-BP398231MTM
CSB-MP398231MTM
CSB-EP398231MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Histone H2A.Z-specific chaperone CHZ1 (CHZ1) CSB-YP398232MTM
CSB-EP398232MTM
CSB-BP398232MTM
CSB-MP398232MTM
CSB-EP398232MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Adenylyltransferase and sulfurtransferase UBA4 (UBA4) CSB-YP398233MTM
CSB-EP398233MTM
CSB-BP398233MTM
CSB-MP398233MTM
CSB-EP398233MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Protein ROT1 (ROT1), partial CSB-YP398234MTM
CSB-EP398234MTM
CSB-BP398234MTM
CSB-MP398234MTM
CSB-EP398234MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii 3-ketoacyl-CoA reductase (PGUG_04787), partial CSB-YP398235MTM1
CSB-EP398235MTM1
CSB-BP398235MTM1
CSB-MP398235MTM1
CSB-EP398235MTM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Autophagy-related protein 11 (ATG11), partial CSB-YP398236MTM
CSB-EP398236MTM
CSB-BP398236MTM
CSB-MP398236MTM
CSB-EP398236MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Potential protein lysine methyltransferase SET5 (SET5) CSB-YP398237MTM
CSB-EP398237MTM
CSB-BP398237MTM
CSB-MP398237MTM
CSB-EP398237MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent RNA helicase MRH4, mitochondrial (MRH4), partial CSB-YP398238MTM
CSB-EP398238MTM
CSB-BP398238MTM
CSB-MP398238MTM
CSB-EP398238MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Golgi apparatus membrane protein TVP23 (TVP23), partial CSB-YP398239MTM1
CSB-EP398239MTM1
CSB-BP398239MTM1
CSB-MP398239MTM1
CSB-EP398239MTM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Signal peptidase complex catalytic subunit SEC11 (SEC11), partial CSB-YP398240LLV
CSB-EP398240LLV
CSB-BP398240LLV
CSB-MP398240LLV
CSB-EP398240LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Probable cysteine protease ATG4 (ATG4), partial CSB-YP398241LLV
CSB-EP398241LLV
CSB-BP398241LLV
CSB-MP398241LLV
CSB-EP398241LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Phosphatidylinositol transfer protein SFH5 (SFH5) CSB-YP398242LLV
CSB-EP398242LLV
CSB-BP398242LLV
CSB-MP398242LLV
CSB-EP398242LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Tethering factor for nuclear proteasome STS1 (STS1) CSB-YP398243LLV
CSB-EP398243LLV
CSB-BP398243LLV
CSB-MP398243LLV
CSB-EP398243LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Nascent polypeptide-associated complex subunit beta (EGD1) CSB-YP398244LLV
CSB-EP398244LLV
CSB-BP398244LLV
CSB-MP398244LLV
CSB-EP398244LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Cap-associated protein CAF20 (CAF20) CSB-YP398245LLV
CSB-EP398245LLV
CSB-BP398245LLV
CSB-MP398245LLV
CSB-EP398245LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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