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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Lodderomyces elongisporus tRNA-dihydrouridine (47) synthase [NAD (P) (+)], partial CSB-YP398246LLV
CSB-EP398246LLV
CSB-BP398246LLV
CSB-MP398246LLV
CSB-EP398246LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Plasma membrane fusion protein PRM1 (PRM1), partial CSB-YP398247LLV1
CSB-EP398247LLV1
CSB-BP398247LLV1
CSB-MP398247LLV1
CSB-EP398247LLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Golgi to ER traffic protein 1 (GET1), partial CSB-YP398248LLV1
CSB-EP398248LLV1
CSB-BP398248LLV1
CSB-MP398248LLV1
CSB-EP398248LLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Protein ROT1 (ROT1), partial CSB-YP398249LLV
CSB-EP398249LLV
CSB-BP398249LLV
CSB-MP398249LLV
CSB-EP398249LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Histone H2A.1 (HTA1) CSB-YP398250LLV
CSB-EP398250LLV
CSB-BP398250LLV
CSB-MP398250LLV
CSB-EP398250LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Amino-acid acetyltransferase, mitochondrial (ARG2) CSB-YP398251LLV
CSB-EP398251LLV
CSB-BP398251LLV
CSB-MP398251LLV
CSB-EP398251LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Histone H2A.2 (HTA2) CSB-YP398252LLV
CSB-EP398252LLV
CSB-BP398252LLV
CSB-MP398252LLV
CSB-EP398252LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus ATP-dependent RNA helicase DED1 (DED1), partial CSB-YP398253LLV
CSB-EP398253LLV
CSB-BP398253LLV
CSB-MP398253LLV
CSB-EP398253LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus ATP-dependent RNA helicase DBP10 (DBP10), partial CSB-YP398254LLV
CSB-EP398254LLV
CSB-BP398254LLV
CSB-MP398254LLV
CSB-EP398254LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Protein RMD9, mitochondrial (RMD9), partial CSB-YP398255LLV
CSB-EP398255LLV
CSB-BP398255LLV
CSB-MP398255LLV
CSB-EP398255LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Nitrogen permease regulator 3 (NPR3), partial CSB-YP398256LLV
CSB-EP398256LLV
CSB-BP398256LLV
CSB-MP398256LLV
CSB-EP398256LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Pre-rRNA-processing protein IPI1 (IPI1) CSB-YP398257LLV
CSB-EP398257LLV
CSB-BP398257LLV
CSB-MP398257LLV
CSB-EP398257LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Vacuolar protein sorting/targeting protein 10 (VPS10), partial CSB-YP398258LLV
CSB-EP398258LLV
CSB-BP398258LLV
CSB-MP398258LLV
CSB-EP398258LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus PAB-dependent poly (A)-specific ribonuclease subunit PAN2, partial CSB-YP398259LLV
CSB-EP398259LLV
CSB-BP398259LLV
CSB-MP398259LLV
CSB-EP398259LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus ATP-dependent rRNA helicase SPB4 (SPB4), partial CSB-YP398260LLV
CSB-EP398260LLV
CSB-BP398260LLV
CSB-MP398260LLV
CSB-EP398260LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus rRNA-processing protein EFG1 (EFG1) CSB-YP398261LLV
CSB-EP398261LLV
CSB-BP398261LLV
CSB-MP398261LLV
CSB-EP398261LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Putative lipase ATG15 (ATG15), partial CSB-YP398262LLV
CSB-EP398262LLV
CSB-BP398262LLV
CSB-MP398262LLV
CSB-EP398262LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Restriction of telomere capping protein 1 (RTC1), partial CSB-YP398263LLV
CSB-EP398263LLV
CSB-BP398263LLV
CSB-MP398263LLV
CSB-EP398263LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Mitochondrial distribution and morphology protein 12 (MDM12), partial CSB-YP398264LLV
CSB-EP398264LLV
CSB-BP398264LLV
CSB-MP398264LLV
CSB-EP398264LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosome maturation factor RimP (rimP) CSB-YP398265BPE
CSB-EP398265BPE
CSB-BP398265BPE
CSB-MP398265BPE
CSB-EP398265BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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