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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium sp. DNA ligase (ligA), partial CSB-YP398306BPE
CSB-EP398306BPE
CSB-BP398306BPE
CSB-MP398306BPE
CSB-EP398306BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP398307BPE
CSB-EP398307BPE
CSB-BP398307BPE
CSB-MP398307BPE
CSB-EP398307BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase (acsF) CSB-YP398308BPE
CSB-EP398308BPE
CSB-BP398308BPE
CSB-MP398308BPE
CSB-EP398308BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Imidazolonepropionase (hutI) CSB-YP398309BPE
CSB-EP398309BPE
CSB-BP398309BPE
CSB-MP398309BPE
CSB-EP398309BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. tRNA (Ile)-lysidine synthase CSB-YP398310BPE
CSB-EP398310BPE
CSB-BP398310BPE
CSB-MP398310BPE
CSB-EP398310BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Urease accessory protein UreE (ureE) CSB-YP398311BPE
CSB-EP398311BPE
CSB-BP398311BPE
CSB-MP398311BPE
CSB-EP398311BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L28 (rpmB) CSB-YP398312BPE
CSB-EP398312BPE
CSB-BP398312BPE
CSB-MP398312BPE
CSB-EP398312BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus 30S ribosomal protein S2 (rpsB) CSB-YP398313DHZ
CSB-EP398313DHZ
CSB-BP398313DHZ
CSB-MP398313DHZ
CSB-EP398313DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP398314DHZ
CSB-EP398314DHZ
CSB-BP398314DHZ
CSB-MP398314DHZ
CSB-EP398314DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus UPF0102 protein DNO_0639 (DNO_0639) CSB-YP398315DHZ
CSB-EP398315DHZ
CSB-BP398315DHZ
CSB-MP398315DHZ
CSB-EP398315DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Putative phosphotransferase DNO_0628 (DNO_0628) CSB-YP398316DHZ
CSB-EP398316DHZ
CSB-BP398316DHZ
CSB-MP398316DHZ
CSB-EP398316DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Ribose-5-phosphate isomerase A (rpiA) CSB-YP398317DHZ
CSB-EP398317DHZ
CSB-BP398317DHZ
CSB-MP398317DHZ
CSB-EP398317DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP398318DHZ
CSB-EP398318DHZ
CSB-BP398318DHZ
CSB-MP398318DHZ
CSB-EP398318DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus tRNA (mo5U34)-methyltransferase CSB-YP398319DHZ
CSB-EP398319DHZ
CSB-BP398319DHZ
CSB-MP398319DHZ
CSB-EP398319DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Acetate kinase (ackA) CSB-YP398320DHZ
CSB-EP398320DHZ
CSB-BP398320DHZ
CSB-MP398320DHZ
CSB-EP398320DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Alanine--tRNA ligase (alaS), partial CSB-YP398321DHZ
CSB-EP398321DHZ
CSB-BP398321DHZ
CSB-MP398321DHZ
CSB-EP398321DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Trigger factor (tig) CSB-YP398322DHZ
CSB-EP398322DHZ
CSB-BP398322DHZ
CSB-MP398322DHZ
CSB-EP398322DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Integration host factor subunit beta (ihfB) CSB-YP398323DHZ
CSB-EP398323DHZ
CSB-BP398323DHZ
CSB-MP398323DHZ
CSB-EP398323DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Isoleucine--tRNA ligase (ileS), partial CSB-YP398324DHZ
CSB-EP398324DHZ
CSB-BP398324DHZ
CSB-MP398324DHZ
CSB-EP398324DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus 50S ribosomal protein L33 (rpmG) CSB-YP398325DHZ
CSB-EP398325DHZ
CSB-BP398325DHZ
CSB-MP398325DHZ
CSB-EP398325DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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