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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium sp. 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP398266BPE
CSB-EP398266BPE
CSB-BP398266BPE
CSB-MP398266BPE
CSB-EP398266BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP398267BPE
CSB-EP398267BPE
CSB-BP398267BPE
CSB-MP398267BPE
CSB-EP398267BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. ATP-dependent protease subunit HslV (hslV) CSB-YP398268BPE
CSB-EP398268BPE
CSB-BP398268BPE
CSB-MP398268BPE
CSB-EP398268BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Putative phosphotransferase BBta_0161 (BBta_0161) CSB-YP398269BPE
CSB-EP398269BPE
CSB-BP398269BPE
CSB-MP398269BPE
CSB-EP398269BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Non-canonical purine NTP pyrophosphatase (BBta_0185) CSB-YP398270BPE
CSB-EP398270BPE
CSB-BP398270BPE
CSB-MP398270BPE
CSB-EP398270BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP398271BPE
CSB-EP398271BPE
CSB-BP398271BPE
CSB-MP398271BPE
CSB-EP398271BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Undecaprenyl-diphosphatase (uppP), partial CSB-YP398272BPE1
CSB-EP398272BPE1
CSB-BP398272BPE1
CSB-MP398272BPE1
CSB-EP398272BPE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Bifunctional protein FolD (folD) CSB-YP398273BPE
CSB-EP398273BPE
CSB-BP398273BPE
CSB-MP398273BPE
CSB-EP398273BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0434 protein BBta_0300 (BBta_0300) CSB-YP398274BPE
CSB-EP398274BPE
CSB-BP398274BPE
CSB-MP398274BPE
CSB-EP398274BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosome maturation factor RimM (rimM) CSB-YP398275BPE
CSB-EP398275BPE
CSB-BP398275BPE
CSB-MP398275BPE
CSB-EP398275BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP398276BPE
CSB-EP398276BPE
CSB-BP398276BPE
CSB-MP398276BPE
CSB-EP398276BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Fructose-1,6-bisphosphatase class 1 1 (fbp1) CSB-YP398277BPE
CSB-EP398277BPE
CSB-BP398277BPE
CSB-MP398277BPE
CSB-EP398277BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Dihydroorotate dehydrogenase (quinone) CSB-YP398278BPE
CSB-EP398278BPE
CSB-BP398278BPE
CSB-MP398278BPE
CSB-EP398278BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Homoserine kinase (thrB) CSB-YP398279BPE
CSB-EP398279BPE
CSB-BP398279BPE
CSB-MP398279BPE
CSB-EP398279BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 60 kDa chaperonin 1 (groL1), partial CSB-YP398280BPE
CSB-EP398280BPE
CSB-BP398280BPE
CSB-MP398280BPE
CSB-EP398280BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Aliphatic amidase (amiE) CSB-YP398281BPE
CSB-EP398281BPE
CSB-BP398281BPE
CSB-MP398281BPE
CSB-EP398281BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP398282BPE
CSB-EP398282BPE
CSB-BP398282BPE
CSB-MP398282BPE
CSB-EP398282BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 60 kDa chaperonin 2 (groL2), partial CSB-YP398283BPE
CSB-EP398283BPE
CSB-BP398283BPE
CSB-MP398283BPE
CSB-EP398283BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 60 kDa chaperonin 3 (groL3), partial CSB-YP398284BPE
CSB-EP398284BPE
CSB-BP398284BPE
CSB-MP398284BPE
CSB-EP398284BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Formyl-coenzyme A transferase (frc) CSB-YP398285BPE
CSB-EP398285BPE
CSB-BP398285BPE
CSB-MP398285BPE
CSB-EP398285BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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