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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio cholerae serotype O1 30S ribosomal protein S17 (rpsQ) CSB-YP399087VEY
CSB-EP399087VEY
CSB-BP399087VEY
CSB-MP399087VEY
CSB-EP399087VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L18 (rplR) CSB-YP399088VEY
CSB-EP399088VEY
CSB-BP399088VEY
CSB-MP399088VEY
CSB-EP399088VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 RNA polymerase-associated protein RapA (rapA), partial CSB-YP399089VEY
CSB-EP399089VEY
CSB-BP399089VEY
CSB-MP399089VEY
CSB-EP399089VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Ribose-5-phosphate isomerase A (rpiA) CSB-YP399090VEY
CSB-EP399090VEY
CSB-BP399090VEY
CSB-MP399090VEY
CSB-EP399090VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Multifunctional CCA protein (cca) CSB-YP399091VEY
CSB-EP399091VEY
CSB-BP399091VEY
CSB-MP399091VEY
CSB-EP399091VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0289 protein VC0395_A2005/VC395_2543 (VC0395_A2005, VC395_2543) CSB-YP399092VEY
CSB-EP399092VEY
CSB-BP399092VEY
CSB-MP399092VEY
CSB-EP399092VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP399093VEY
CSB-EP399093VEY
CSB-BP399093VEY
CSB-MP399093VEY
CSB-EP399093VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Homoserine kinase (thrB) CSB-YP399094VEY
CSB-EP399094VEY
CSB-BP399094VEY
CSB-MP399094VEY
CSB-EP399094VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Phosphopentomutase (deoB) CSB-YP399095VEY
CSB-EP399095VEY
CSB-BP399095VEY
CSB-MP399095VEY
CSB-EP399095VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Na (+)-translocating NADH-quinone reductase subunit F, partial CSB-YP399096VEY
CSB-EP399096VEY
CSB-BP399096VEY
CSB-MP399096VEY
CSB-EP399096VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Ribosomal protein S6 modification protein (rimK) CSB-YP399097VEY
CSB-EP399097VEY
CSB-BP399097VEY
CSB-MP399097VEY
CSB-EP399097VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0255 protein VC0395_A1866/VC395_2392 (VC0395_A1866, VC395_2392) CSB-YP399098VEY
CSB-EP399098VEY
CSB-BP399098VEY
CSB-MP399098VEY
CSB-EP399098VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP399099VEY
CSB-EP399099VEY
CSB-BP399099VEY
CSB-MP399099VEY
CSB-EP399099VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP399100VEY
CSB-EP399100VEY
CSB-BP399100VEY
CSB-MP399100VEY
CSB-EP399100VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Flagellin C (flaC) CSB-YP399101VEY
CSB-EP399101VEY
CSB-BP399101VEY
CSB-MP399101VEY
CSB-EP399101VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP399102VEY
CSB-EP399102VEY
CSB-BP399102VEY
CSB-MP399102VEY
CSB-EP399102VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0115 protein VC0395_A1701/VC395_2233 (VC0395_A1701, VC395_2233) CSB-YP399103VEY
CSB-EP399103VEY
CSB-BP399103VEY
CSB-MP399103VEY
CSB-EP399103VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Nucleoid-associated protein VC0395_A1624/VC395_2154 (VC0395_A1624, VC395_2154) CSB-YP399104VEY
CSB-EP399104VEY
CSB-BP399104VEY
CSB-MP399104VEY
CSB-EP399104VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0745 protein VC0395_A1544/VC395_2072 (VC0395_A1544, VC395_2072) CSB-YP399105VEY
CSB-EP399105VEY
CSB-BP399105VEY
CSB-MP399105VEY
CSB-EP399105VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0227 protein VC0395_A1482/VC395_2007 (VC0395_A1482, VC395_2007) CSB-YP399106VEY
CSB-EP399106VEY
CSB-BP399106VEY
CSB-MP399106VEY
CSB-EP399106VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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