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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Vibrio cholerae serotype O1 Tryptophanase (tnaA) CSB-YP399047VEY
CSB-EP399047VEY
CSB-BP399047VEY
CSB-MP399047VEY
CSB-EP399047VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Lipase chaperone (lifO), partial CSB-YP399048VEY
CSB-EP399048VEY
CSB-BP399048VEY
CSB-MP399048VEY
CSB-EP399048VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L20 (rplT) CSB-YP399049VEY
CSB-EP399049VEY
CSB-BP399049VEY
CSB-MP399049VEY
CSB-EP399049VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Glycerol kinase (glpK), partial CSB-YP399050VEY
CSB-EP399050VEY
CSB-BP399050VEY
CSB-MP399050VEY
CSB-EP399050VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Methylglyoxal synthase (mgsA) CSB-YP399051VEY
CSB-EP399051VEY
CSB-BP399051VEY
CSB-MP399051VEY
CSB-EP399051VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 H (+)/Cl (-) exchange transporter ClcA, partial CSB-YP399052VEY
CSB-EP399052VEY
CSB-BP399052VEY
CSB-MP399052VEY
CSB-EP399052VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Putative phosphotransferase VC0395_0252/VC395_A1010 (VC0395_0252, VC395_A1010) CSB-YP399053VEY
CSB-EP399053VEY
CSB-BP399053VEY
CSB-MP399053VEY
CSB-EP399053VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP399054VEY
CSB-EP399054VEY
CSB-BP399054VEY
CSB-MP399054VEY
CSB-EP399054VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Glucans biosynthesis protein G (opgG), partial CSB-YP399055VEY
CSB-EP399055VEY
CSB-BP399055VEY
CSB-MP399055VEY
CSB-EP399055VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Formimidoylglutamase (hutG) CSB-YP399056VEY
CSB-EP399056VEY
CSB-BP399056VEY
CSB-MP399056VEY
CSB-EP399056VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Anthranilate phosphoribosyltransferase (trpD) CSB-YP399057VEY
CSB-EP399057VEY
CSB-BP399057VEY
CSB-MP399057VEY
CSB-EP399057VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP399058VEY
CSB-EP399058VEY
CSB-BP399058VEY
CSB-MP399058VEY
CSB-EP399058VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Biotin synthase (bioB) CSB-YP399059VEY
CSB-EP399059VEY
CSB-BP399059VEY
CSB-MP399059VEY
CSB-EP399059VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Methionine--tRNA ligase (metG), partial CSB-YP399060VEY
CSB-EP399060VEY
CSB-BP399060VEY
CSB-MP399060VEY
CSB-EP399060VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Electron transport complex protein RnfB (rnfB) CSB-YP399061VEY
CSB-EP399061VEY
CSB-BP399061VEY
CSB-MP399061VEY
CSB-EP399061VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Protein CysZ homolog (cysZ), partial CSB-YP399062VEY1
CSB-EP399062VEY1
CSB-BP399062VEY1
CSB-MP399062VEY1
CSB-EP399062VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Probable rRNA maturation factor (VC0395_A0482, VC395_0975) CSB-YP399063VEY
CSB-EP399063VEY
CSB-BP399063VEY
CSB-MP399063VEY
CSB-EP399063VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP399064VEY
CSB-EP399064VEY
CSB-BP399064VEY
CSB-MP399064VEY
CSB-EP399064VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Protein grpE (grpE) CSB-YP399065VEY
CSB-EP399065VEY
CSB-BP399065VEY
CSB-MP399065VEY
CSB-EP399065VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Ribosome association toxin RatA (ratA) CSB-YP399066VEY
CSB-EP399066VEY
CSB-BP399066VEY
CSB-MP399066VEY
CSB-EP399066VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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