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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L32 (rpmF) CSB-YP401447FDT
CSB-EP401447FDT
CSB-BP401447FDT
CSB-MP401447FDT
CSB-EP401447FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP401448FDT
CSB-EP401448FDT
CSB-BP401448FDT
CSB-MP401448FDT
CSB-EP401448FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Adenylate kinase (adk) CSB-YP401449FDT
CSB-EP401449FDT
CSB-BP401449FDT
CSB-MP401449FDT
CSB-EP401449FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 30S ribosomal protein S12 (rpsL) CSB-YP401450FDT
CSB-EP401450FDT
CSB-BP401450FDT
CSB-MP401450FDT
CSB-EP401450FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L36 (rpmJ) CSB-YP401451FDT
CSB-EP401451FDT
CSB-BP401451FDT
CSB-MP401451FDT
CSB-EP401451FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Enolase (eno) CSB-YP401452FDT
CSB-EP401452FDT
CSB-BP401452FDT
CSB-MP401452FDT
CSB-EP401452FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. UPF0296 protein DehaBAV1_0033 (DehaBAV1_0033) CSB-YP401453DHU
CSB-EP401453DHU
CSB-BP401453DHU
CSB-MP401453DHU
CSB-EP401453DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP401454DHU
CSB-EP401454DHU
CSB-BP401454DHU
CSB-MP401454DHU
CSB-EP401454DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. UPF0145 protein DehaBAV1_1363 (DehaBAV1_1363) CSB-YP401455DHU
CSB-EP401455DHU
CSB-BP401455DHU
CSB-MP401455DHU
CSB-EP401455DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 60 kDa chaperonin (groL), partial CSB-YP401456DHU
CSB-EP401456DHU
CSB-BP401456DHU
CSB-MP401456DHU
CSB-EP401456DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP401457DHU
CSB-EP401457DHU
CSB-BP401457DHU
CSB-MP401457DHU
CSB-EP401457DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP401458DHU
CSB-EP401458DHU
CSB-BP401458DHU
CSB-MP401458DHU
CSB-EP401458DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Aspartate carbamoyltransferase (pyrB) CSB-YP401459DHU
CSB-EP401459DHU
CSB-BP401459DHU
CSB-MP401459DHU
CSB-EP401459DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 50S ribosomal protein L33 (rpmG) CSB-YP401460DHU
CSB-EP401460DHU
CSB-BP401460DHU
CSB-MP401460DHU
CSB-EP401460DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Dihydrodipicolinate synthase (dapA) CSB-YP401461DHU
CSB-EP401461DHU
CSB-BP401461DHU
CSB-MP401461DHU
CSB-EP401461DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Histidinol-phosphate aminotransferase (hisC) CSB-YP401462DHU
CSB-EP401462DHU
CSB-BP401462DHU
CSB-MP401462DHU
CSB-EP401462DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Pantothenate synthetase (panC) CSB-YP401463DHU
CSB-EP401463DHU
CSB-BP401463DHU
CSB-MP401463DHU
CSB-EP401463DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Phosphomethylpyrimidine synthase (thiC) CSB-YP401464DHU
CSB-EP401464DHU
CSB-BP401464DHU
CSB-MP401464DHU
CSB-EP401464DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 50S ribosomal protein L35 (rpmI) CSB-YP401465DHU
CSB-EP401465DHU
CSB-BP401465DHU
CSB-MP401465DHU
CSB-EP401465DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP401466DHU
CSB-EP401466DHU
CSB-BP401466DHU
CSB-MP401466DHU
CSB-EP401466DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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