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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acidiphilium cryptum Glycogen synthase (glgA) CSB-YP401847ATZ
CSB-EP401847ATZ
CSB-BP401847ATZ
CSB-MP401847ATZ
CSB-EP401847ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L21 (rplU) CSB-YP401848ATZ
CSB-EP401848ATZ
CSB-BP401848ATZ
CSB-MP401848ATZ
CSB-EP401848ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Probable acetoacetate decarboxylase (adc) CSB-YP401849ATZ
CSB-EP401849ATZ
CSB-BP401849ATZ
CSB-MP401849ATZ
CSB-EP401849ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Aspartate carbamoyltransferase (pyrB) CSB-YP401850ATZ
CSB-EP401850ATZ
CSB-BP401850ATZ
CSB-MP401850ATZ
CSB-EP401850ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP401851ATZ
CSB-EP401851ATZ
CSB-BP401851ATZ
CSB-MP401851ATZ
CSB-EP401851ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Transcription elongation factor GreA (greA) CSB-YP401852ATZ
CSB-EP401852ATZ
CSB-BP401852ATZ
CSB-MP401852ATZ
CSB-EP401852ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Proline--tRNA ligase (proS) CSB-YP401853ATZ
CSB-EP401853ATZ
CSB-BP401853ATZ
CSB-MP401853ATZ
CSB-EP401853ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP401854ATZ
CSB-EP401854ATZ
CSB-BP401854ATZ
CSB-MP401854ATZ
CSB-EP401854ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP401855ATZ1
CSB-EP401855ATZ1
CSB-BP401855ATZ1
CSB-MP401855ATZ1
CSB-EP401855ATZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Chorismate synthase (aroC) CSB-YP401856ATZ
CSB-EP401856ATZ
CSB-BP401856ATZ
CSB-MP401856ATZ
CSB-EP401856ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Cysteine--tRNA ligase (cysS) CSB-YP401857ATZ
CSB-EP401857ATZ
CSB-BP401857ATZ
CSB-MP401857ATZ
CSB-EP401857ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP401858ATZ
CSB-EP401858ATZ
CSB-BP401858ATZ
CSB-MP401858ATZ
CSB-EP401858ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP401859ATZ
CSB-EP401859ATZ
CSB-BP401859ATZ
CSB-MP401859ATZ
CSB-EP401859ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP401860ATZ
CSB-EP401860ATZ
CSB-BP401860ATZ
CSB-MP401860ATZ
CSB-EP401860ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L18 (rplR) CSB-YP401861ATZ
CSB-EP401861ATZ
CSB-BP401861ATZ
CSB-MP401861ATZ
CSB-EP401861ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L22 (rplV) CSB-YP401862ATZ
CSB-EP401862ATZ
CSB-BP401862ATZ
CSB-MP401862ATZ
CSB-EP401862ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 30S ribosomal protein S10 (rpsJ) CSB-YP401863ATZ
CSB-EP401863ATZ
CSB-BP401863ATZ
CSB-MP401863ATZ
CSB-EP401863ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L1 (rplA) CSB-YP401864ATZ
CSB-EP401864ATZ
CSB-BP401864ATZ
CSB-MP401864ATZ
CSB-EP401864ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Leucine--tRNA ligase (leuS), partial CSB-YP401865ATZ
CSB-EP401865ATZ
CSB-BP401865ATZ
CSB-MP401865ATZ
CSB-EP401865ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP401866ATZ
CSB-EP401866ATZ
CSB-BP401866ATZ
CSB-MP401866ATZ
CSB-EP401866ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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