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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter uraniireducens Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP400447GEI
CSB-EP400447GEI
CSB-BP400447GEI
CSB-MP400447GEI
CSB-EP400447GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Acylphosphatase (acyP) CSB-YP400448GEI
CSB-EP400448GEI
CSB-BP400448GEI
CSB-MP400448GEI
CSB-EP400448GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Bifunctional protein FolD (folD) CSB-YP400449GEI
CSB-EP400449GEI
CSB-BP400449GEI
CSB-MP400449GEI
CSB-EP400449GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 30S ribosomal protein S4 (rpsD) CSB-YP400450GEI
CSB-EP400450GEI
CSB-BP400450GEI
CSB-MP400450GEI
CSB-EP400450GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L30 (rpmD) CSB-YP400451GEI
CSB-EP400451GEI
CSB-BP400451GEI
CSB-MP400451GEI
CSB-EP400451GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L2 (rplB) CSB-YP400452GEI
CSB-EP400452GEI
CSB-BP400452GEI
CSB-MP400452GEI
CSB-EP400452GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L11 (rplK) CSB-YP400453GEI
CSB-EP400453GEI
CSB-BP400453GEI
CSB-MP400453GEI
CSB-EP400453GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP400454GEI
CSB-EP400454GEI
CSB-BP400454GEI
CSB-MP400454GEI
CSB-EP400454GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Glycogen synthase (glgA) CSB-YP400455GEI
CSB-EP400455GEI
CSB-BP400455GEI
CSB-MP400455GEI
CSB-EP400455GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Probable transaldolase (tal) CSB-YP400456GEI
CSB-EP400456GEI
CSB-BP400456GEI
CSB-MP400456GEI
CSB-EP400456GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP400457GEI
CSB-EP400457GEI
CSB-BP400457GEI
CSB-MP400457GEI
CSB-EP400457GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UPF0502 protein Gura_0277 (Gura_0277) CSB-YP400458GEI
CSB-EP400458GEI
CSB-BP400458GEI
CSB-MP400458GEI
CSB-EP400458GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Alanine--tRNA ligase (alaS), partial CSB-YP400459GEI
CSB-EP400459GEI
CSB-BP400459GEI
CSB-MP400459GEI
CSB-EP400459GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens tRNA dimethylallyltransferase 1 (miaA1) CSB-YP400460GEI
CSB-EP400460GEI
CSB-BP400460GEI
CSB-MP400460GEI
CSB-EP400460GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Protein translocase subunit SecA (secA), partial CSB-YP400461GEI
CSB-EP400461GEI
CSB-BP400461GEI
CSB-MP400461GEI
CSB-EP400461GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP400462GEI
CSB-EP400462GEI
CSB-BP400462GEI
CSB-MP400462GEI
CSB-EP400462GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig UPF0549 protein C20orf43 homolog CSB-YP400463PI
CSB-EP400463PI
CSB-BP400463PI
CSB-MP400463PI
CSB-EP400463PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP400464SVC
CSB-EP400464SVC
CSB-BP400464SVC
CSB-MP400464SVC
CSB-EP400464SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L35 (rpmI) CSB-YP400465SVC
CSB-EP400465SVC
CSB-BP400465SVC
CSB-MP400465SVC
CSB-EP400465SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem I iron-sulfur center (psaC) CSB-YP400466SVC
CSB-EP400466SVC
CSB-BP400466SVC
CSB-MP400466SVC
CSB-EP400466SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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