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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum UPF0182 protein CBO0011/CLC_0020 (CBO0011, CLC_0020), partial CSB-YP400527CWV
CSB-EP400527CWV
CSB-BP400527CWV
CSB-MP400527CWV
CSB-EP400527CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Sugar fermentation stimulation protein homolog (sfsA) CSB-YP400528CWV
CSB-EP400528CWV
CSB-BP400528CWV
CSB-MP400528CWV
CSB-EP400528CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Peptidase T (pepT) CSB-YP400529CWV
CSB-EP400529CWV
CSB-BP400529CWV
CSB-MP400529CWV
CSB-EP400529CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Asparagine--tRNA ligase (asnS) CSB-YP400530CWV
CSB-EP400530CWV
CSB-BP400530CWV
CSB-MP400530CWV
CSB-EP400530CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable transaldolase (tal) CSB-YP400531CWV
CSB-EP400531CWV
CSB-BP400531CWV
CSB-MP400531CWV
CSB-EP400531CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP400532CWV
CSB-EP400532CWV
CSB-BP400532CWV
CSB-MP400532CWV
CSB-EP400532CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Cytidylate kinase (cmk) CSB-YP400533CWV
CSB-EP400533CWV
CSB-BP400533CWV
CSB-MP400533CWV
CSB-EP400533CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP400534CWV
CSB-EP400534CWV
CSB-BP400534CWV
CSB-MP400534CWV
CSB-EP400534CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP400535CWV
CSB-EP400535CWV
CSB-BP400535CWV
CSB-MP400535CWV
CSB-EP400535CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP400536CWV
CSB-EP400536CWV
CSB-BP400536CWV
CSB-MP400536CWV
CSB-EP400536CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosome-binding factor A (rbfA) CSB-YP400537CWV
CSB-EP400537CWV
CSB-BP400537CWV
CSB-MP400537CWV
CSB-EP400537CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S2 (rpsB) CSB-YP400538CWV
CSB-EP400538CWV
CSB-BP400538CWV
CSB-MP400538CWV
CSB-EP400538CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA (guanine-N (1)-)-methyltransferase CSB-YP400539CWV
CSB-EP400539CWV
CSB-BP400539CWV
CSB-MP400539CWV
CSB-EP400539CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP400540CWV
CSB-EP400540CWV
CSB-BP400540CWV
CSB-MP400540CWV
CSB-EP400540CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0297 protein CBO2563/CLC_2434 (CBO2563, CLC_2434) CSB-YP400541CWV
CSB-EP400541CWV
CSB-BP400541CWV
CSB-MP400541CWV
CSB-EP400541CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum V-type ATP synthase beta chain (atpB) CSB-YP400542CWV
CSB-EP400542CWV
CSB-BP400542CWV
CSB-MP400542CWV
CSB-EP400542CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP400543CWV
CSB-EP400543CWV
CSB-BP400543CWV
CSB-MP400543CWV
CSB-EP400543CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable rRNA maturation factor (CBO2949, CLC_2844) CSB-YP400544CWV
CSB-EP400544CWV
CSB-BP400544CWV
CSB-MP400544CWV
CSB-EP400544CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP400545CWV
CSB-EP400545CWV
CSB-BP400545CWV
CSB-MP400545CWV
CSB-EP400545CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Aspartate--tRNA ligase (aspS), partial CSB-YP400546CWV
CSB-EP400546CWV
CSB-BP400546CWV
CSB-MP400546CWV
CSB-EP400546CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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