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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dehalococcoides sp. LL-diaminopimelate aminotransferase (dapL) CSB-YP400747DHU
CSB-EP400747DHU
CSB-BP400747DHU
CSB-MP400747DHU
CSB-EP400747DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP400748DHU
CSB-EP400748DHU
CSB-BP400748DHU
CSB-MP400748DHU
CSB-EP400748DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Serine--tRNA ligase (serS) CSB-YP400749DHU
CSB-EP400749DHU
CSB-BP400749DHU
CSB-MP400749DHU
CSB-EP400749DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. ATP synthase subunit c (atpE), partial CSB-YP400750DHU1
CSB-EP400750DHU1
CSB-BP400750DHU1
CSB-MP400750DHU1
CSB-EP400750DHU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 50S ribosomal protein L17 (rplQ) CSB-YP400751DHU
CSB-EP400751DHU
CSB-BP400751DHU
CSB-MP400751DHU
CSB-EP400751DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 30S ribosomal protein S14 type Z (rpsZ) CSB-YP400752DHU
CSB-EP400752DHU
CSB-BP400752DHU
CSB-MP400752DHU
CSB-EP400752DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 30S ribosomal protein S3 (rpsC) CSB-YP400753DHU
CSB-EP400753DHU
CSB-BP400753DHU
CSB-MP400753DHU
CSB-EP400753DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP400754DHU
CSB-EP400754DHU
CSB-BP400754DHU
CSB-MP400754DHU
CSB-EP400754DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 30S ribosomal protein S21 (rpsU) CSB-YP400755DHU
CSB-EP400755DHU
CSB-BP400755DHU
CSB-MP400755DHU
CSB-EP400755DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. DNA polymerase IV (dinB) CSB-YP400756DHU
CSB-EP400756DHU
CSB-BP400756DHU
CSB-MP400756DHU
CSB-EP400756DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP400757ATZ
CSB-EP400757ATZ
CSB-BP400757ATZ
CSB-MP400757ATZ
CSB-EP400757ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum UPF0262 protein Acry_0160 (Acry_0160) CSB-YP400758ATZ
CSB-EP400758ATZ
CSB-BP400758ATZ
CSB-MP400758ATZ
CSB-EP400758ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum GTPase obg (obg) CSB-YP400759ATZ
CSB-EP400759ATZ
CSB-BP400759ATZ
CSB-MP400759ATZ
CSB-EP400759ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP400760ATZ
CSB-EP400760ATZ
CSB-BP400760ATZ
CSB-MP400760ATZ
CSB-EP400760ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP400761ATZ1
CSB-EP400761ATZ1
CSB-BP400761ATZ1
CSB-MP400761ATZ1
CSB-EP400761ATZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP400762ATZ
CSB-EP400762ATZ
CSB-BP400762ATZ
CSB-MP400762ATZ
CSB-EP400762ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP400763ATZ
CSB-EP400763ATZ
CSB-BP400763ATZ
CSB-MP400763ATZ
CSB-EP400763ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP400764ATZ
CSB-EP400764ATZ
CSB-BP400764ATZ
CSB-MP400764ATZ
CSB-EP400764ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Trigger factor (tig) CSB-YP400765ATZ
CSB-EP400765ATZ
CSB-BP400765ATZ
CSB-MP400765ATZ
CSB-EP400765ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP400766ATZ1
CSB-EP400766ATZ1
CSB-BP400766ATZ1
CSB-MP400766ATZ1
CSB-EP400766ATZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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