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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Flavobacterium johnsoniae 5'-nucleotidase surE (surE) CSB-YP400727FDT
CSB-EP400727FDT
CSB-BP400727FDT
CSB-MP400727FDT
CSB-EP400727FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Aspartate--tRNA ligase (aspS), partial CSB-YP400728FDT
CSB-EP400728FDT
CSB-BP400728FDT
CSB-MP400728FDT
CSB-EP400728FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP400729FDT
CSB-EP400729FDT
CSB-BP400729FDT
CSB-MP400729FDT
CSB-EP400729FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L3 (rplC) CSB-YP400730FDT
CSB-EP400730FDT
CSB-BP400730FDT
CSB-MP400730FDT
CSB-EP400730FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L16 (rplP) CSB-YP400731FDT
CSB-EP400731FDT
CSB-BP400731FDT
CSB-MP400731FDT
CSB-EP400731FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L30 (rpmD) CSB-YP400732FDT
CSB-EP400732FDT
CSB-BP400732FDT
CSB-MP400732FDT
CSB-EP400732FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP400733FDT
CSB-EP400733FDT
CSB-BP400733FDT
CSB-MP400733FDT
CSB-EP400733FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Ribonuclease Y (rny), partial CSB-YP400734FDT
CSB-EP400734FDT
CSB-BP400734FDT
CSB-MP400734FDT
CSB-EP400734FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. GTPase obg (obg) CSB-YP400735DHU
CSB-EP400735DHU
CSB-BP400735DHU
CSB-MP400735DHU
CSB-EP400735DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Cysteine--tRNA ligase (cysS) CSB-YP400736DHU
CSB-EP400736DHU
CSB-BP400736DHU
CSB-MP400736DHU
CSB-EP400736DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Quinolinate synthase A (nadA) CSB-YP400737DHU
CSB-EP400737DHU
CSB-BP400737DHU
CSB-MP400737DHU
CSB-EP400737DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Lipoprotein signal peptidase (lspA), partial CSB-YP400738DHU1
CSB-EP400738DHU1
CSB-BP400738DHU1
CSB-MP400738DHU1
CSB-EP400738DHU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP400739DHU
CSB-EP400739DHU
CSB-BP400739DHU
CSB-MP400739DHU
CSB-EP400739DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Glutamate 5-kinase (proB) CSB-YP400740DHU
CSB-EP400740DHU
CSB-BP400740DHU
CSB-MP400740DHU
CSB-EP400740DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Maf-like protein DehaBAV1_0949 (DehaBAV1_0949) CSB-YP400741DHU
CSB-EP400741DHU
CSB-BP400741DHU
CSB-MP400741DHU
CSB-EP400741DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 50S ribosomal protein L11 (rplK) CSB-YP400742DHU
CSB-EP400742DHU
CSB-BP400742DHU
CSB-MP400742DHU
CSB-EP400742DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Dihydrodipicolinate reductase (dapB) CSB-YP400743DHU
CSB-EP400743DHU
CSB-BP400743DHU
CSB-MP400743DHU
CSB-EP400743DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP400744DHU
CSB-EP400744DHU
CSB-BP400744DHU
CSB-MP400744DHU
CSB-EP400744DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP400745DHU
CSB-EP400745DHU
CSB-BP400745DHU
CSB-MP400745DHU
CSB-EP400745DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP400746DHU
CSB-EP400746DHU
CSB-BP400746DHU
CSB-MP400746DHU
CSB-EP400746DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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