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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidiphilium cryptum 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP401867ATZ
CSB-EP401867ATZ
CSB-BP401867ATZ
CSB-MP401867ATZ
CSB-EP401867ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP401868ATZ
CSB-EP401868ATZ
CSB-BP401868ATZ
CSB-MP401868ATZ
CSB-EP401868ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum 50S ribosomal protein L36 (rpmJ) CSB-YP401869ATZ
CSB-EP401869ATZ
CSB-BP401869ATZ
CSB-MP401869ATZ
CSB-EP401869ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Shikimate dehydrogenase (aroE) CSB-YP401870ATZ
CSB-EP401870ATZ
CSB-BP401870ATZ
CSB-MP401870ATZ
CSB-EP401870ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum LexA repressor (lexA) CSB-YP401871ATZ
CSB-EP401871ATZ
CSB-BP401871ATZ
CSB-MP401871ATZ
CSB-EP401871ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum L-rhamnose mutarotase (rhaM) CSB-YP401872ATZ
CSB-EP401872ATZ
CSB-BP401872ATZ
CSB-MP401872ATZ
CSB-EP401872ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP401873GEI
CSB-EP401873GEI
CSB-BP401873GEI
CSB-MP401873GEI
CSB-EP401873GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Glutamate--tRNA ligase (gltX) CSB-YP401874GEI
CSB-EP401874GEI
CSB-BP401874GEI
CSB-MP401874GEI
CSB-EP401874GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Flavoprotein wrbA (wrbA) CSB-YP401875GEI
CSB-EP401875GEI
CSB-BP401875GEI
CSB-MP401875GEI
CSB-EP401875GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UPF0502 protein Gura_3445 (Gura_3445) CSB-YP401876GEI
CSB-EP401876GEI
CSB-BP401876GEI
CSB-MP401876GEI
CSB-EP401876GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 30S ribosomal protein S6 (rpsF) CSB-YP401877GEI
CSB-EP401877GEI
CSB-BP401877GEI
CSB-MP401877GEI
CSB-EP401877GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Dihydroxy-acid dehydratase (ilvD), partial CSB-YP401878GEI
CSB-EP401878GEI
CSB-BP401878GEI
CSB-MP401878GEI
CSB-EP401878GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L19 (rplS) CSB-YP401879GEI
CSB-EP401879GEI
CSB-BP401879GEI
CSB-MP401879GEI
CSB-EP401879GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP401880GEI
CSB-EP401880GEI
CSB-BP401880GEI
CSB-MP401880GEI
CSB-EP401880GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 30S ribosomal protein S21 (rpsU) CSB-YP401881GEI
CSB-EP401881GEI
CSB-BP401881GEI
CSB-MP401881GEI
CSB-EP401881GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Carbon storage regulator homolog (csrA) CSB-YP401882GEI
CSB-EP401882GEI
CSB-BP401882GEI
CSB-MP401882GEI
CSB-EP401882GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP401883GEI
CSB-EP401883GEI
CSB-BP401883GEI
CSB-MP401883GEI
CSB-EP401883GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 60 kDa chaperonin (groL), partial CSB-YP401884GEI
CSB-EP401884GEI
CSB-BP401884GEI
CSB-MP401884GEI
CSB-EP401884GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP401885GEI
CSB-EP401885GEI
CSB-BP401885GEI
CSB-MP401885GEI
CSB-EP401885GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Ribonuclease P protein component (rnpA) CSB-YP401886GEI
CSB-EP401886GEI
CSB-BP401886GEI
CSB-MP401886GEI
CSB-EP401886GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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