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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Serine--tRNA ligase (serS) CSB-YP402068SUJ
CSB-EP402068SUJ
CSB-BP402068SUJ
CSB-MP402068SUJ
CSB-EP402068SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP402069SUJ
CSB-EP402069SUJ
CSB-BP402069SUJ
CSB-MP402069SUJ
CSB-EP402069SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Antiholin-like protein LrgA (lrgA), partial CSB-YP402070SUJ1
CSB-EP402070SUJ1
CSB-BP402070SUJ1
CSB-MP402070SUJ1
CSB-EP402070SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Xanthine phosphoribosyltransferase (xpt) CSB-YP402071SUJ
CSB-EP402071SUJ
CSB-BP402071SUJ
CSB-MP402071SUJ
CSB-EP402071SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP402072SUJ
CSB-EP402072SUJ
CSB-BP402072SUJ
CSB-MP402072SUJ
CSB-EP402072SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP402073SUJ
CSB-EP402073SUJ
CSB-BP402073SUJ
CSB-MP402073SUJ
CSB-EP402073SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTP cyclohydrolase folE2 (folE2) CSB-YP402074SUJ
CSB-EP402074SUJ
CSB-BP402074SUJ
CSB-MP402074SUJ
CSB-EP402074SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arginine--tRNA ligase (argS), partial CSB-YP402075SUJ
CSB-EP402075SUJ
CSB-BP402075SUJ
CSB-MP402075SUJ
CSB-EP402075SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Response regulator protein graR (graR) CSB-YP402076SUJ
CSB-EP402076SUJ
CSB-BP402076SUJ
CSB-MP402076SUJ
CSB-EP402076SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP402077SUJ
CSB-EP402077SUJ
CSB-BP402077SUJ
CSB-MP402077SUJ
CSB-EP402077SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoglycerate kinase (pgk) CSB-YP402078SUJ
CSB-EP402078SUJ
CSB-BP402078SUJ
CSB-MP402078SUJ
CSB-EP402078SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit E1, partial CSB-YP402079SUJ1
CSB-EP402079SUJ1
CSB-BP402079SUJ1
CSB-MP402079SUJ1
CSB-EP402079SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit B1, partial CSB-YP402080SUJ1
CSB-EP402080SUJ1
CSB-BP402080SUJ1
CSB-MP402080SUJ1
CSB-EP402080SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Coenzyme A disulfide reductase (cdr) CSB-YP402081SUJ
CSB-EP402081SUJ
CSB-BP402081SUJ
CSB-MP402081SUJ
CSB-EP402081SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide chain release factor 3 (prfC), partial CSB-YP402082SUJ
CSB-EP402082SUJ
CSB-BP402082SUJ
CSB-MP402082SUJ
CSB-EP402082SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Bifunctional purine biosynthesis protein PurH (purH) CSB-YP402083SUJ
CSB-EP402083SUJ
CSB-BP402083SUJ
CSB-MP402083SUJ
CSB-EP402083SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0298 protein SaurJH9_1181 (SaurJH9_1181) CSB-YP402084SUJ
CSB-EP402084SUJ
CSB-BP402084SUJ
CSB-MP402084SUJ
CSB-EP402084SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UvrABC system protein C (uvrC), partial CSB-YP402085SUJ
CSB-EP402085SUJ
CSB-BP402085SUJ
CSB-MP402085SUJ
CSB-EP402085SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Isoleucine--tRNA ligase (ileS), partial CSB-YP402086SUJ
CSB-EP402086SUJ
CSB-BP402086SUJ
CSB-MP402086SUJ
CSB-EP402086SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP402087SUJ
CSB-EP402087SUJ
CSB-BP402087SUJ
CSB-MP402087SUJ
CSB-EP402087SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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