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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae subsp. pneumoniae 30S ribosomal protein S13 (rpsM) CSB-YP413333KAX
CSB-EP413333KAX
CSB-BP413333KAX
CSB-MP413333KAX
CSB-EP413333KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L6 (rplF) CSB-YP413334KAX
CSB-EP413334KAX
CSB-BP413334KAX
CSB-MP413334KAX
CSB-EP413334KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L4 (rplD) CSB-YP413335KAX
CSB-EP413335KAX
CSB-BP413335KAX
CSB-MP413335KAX
CSB-EP413335KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 30S ribosomal protein S10 (rpsJ) CSB-YP413336KAX
CSB-EP413336KAX
CSB-BP413336KAX
CSB-MP413336KAX
CSB-EP413336KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Sulfurtransferase TusD (tusD) CSB-YP413337KAX
CSB-EP413337KAX
CSB-BP413337KAX
CSB-MP413337KAX
CSB-EP413337KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP413338KAX
CSB-EP413338KAX
CSB-BP413338KAX
CSB-MP413338KAX
CSB-EP413338KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP413339KAX
CSB-EP413339KAX
CSB-BP413339KAX
CSB-MP413339KAX
CSB-EP413339KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Nickel-responsive regulator (nikR) CSB-YP413340KAX
CSB-EP413340KAX
CSB-BP413340KAX
CSB-MP413340KAX
CSB-EP413340KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP413341KAX
CSB-EP413341KAX
CSB-BP413341KAX
CSB-MP413341KAX
CSB-EP413341KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Phosphopantetheine adenylyltransferase (coaD) CSB-YP413342KAX
CSB-EP413342KAX
CSB-BP413342KAX
CSB-MP413342KAX
CSB-EP413342KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Phosphonoacetaldehyde hydrolase (phnX) CSB-YP413343KAX
CSB-EP413343KAX
CSB-BP413343KAX
CSB-MP413343KAX
CSB-EP413343KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae ATP synthase epsilon chain (atpC) CSB-YP413344KAX
CSB-EP413344KAX
CSB-BP413344KAX
CSB-MP413344KAX
CSB-EP413344KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP413345KAX
CSB-EP413345KAX
CSB-BP413345KAX
CSB-MP413345KAX
CSB-EP413345KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae HTH-type transcriptional activator rhaS (rhaS) CSB-YP413346KAX
CSB-EP413346KAX
CSB-BP413346KAX
CSB-MP413346KAX
CSB-EP413346KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Protein CyaY (cyaY) CSB-YP413347KAX
CSB-EP413347KAX
CSB-BP413347KAX
CSB-MP413347KAX
CSB-EP413347KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L1 (rplA) CSB-YP413348KAX
CSB-EP413348KAX
CSB-BP413348KAX
CSB-MP413348KAX
CSB-EP413348KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae LexA repressor (lexA) CSB-YP413349KAX
CSB-EP413349KAX
CSB-BP413349KAX
CSB-MP413349KAX
CSB-EP413349KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 60 kDa chaperonin (groL), partial CSB-YP413350KAX
CSB-BP413350KAX
CSB-MP413350KAX
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP413351KAX
CSB-EP413351KAX
CSB-BP413351KAX
CSB-MP413351KAX
CSB-EP413351KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Primosomal protein 1 (dnaT) CSB-YP413352KAX
CSB-EP413352KAX
CSB-BP413352KAX
CSB-MP413352KAX
CSB-EP413352KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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