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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Transcriptional repressor NrdR (nrdR) CSB-YP413414SUI
CSB-EP413414SUI
CSB-BP413414SUI
CSB-MP413414SUI
CSB-EP413414SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetate kinase (ackA) CSB-YP413415SUI
CSB-EP413415SUI
CSB-BP413415SUI
CSB-MP413415SUI
CSB-EP413415SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP413416SUI
CSB-EP413416SUI
CSB-BP413416SUI
CSB-MP413416SUI
CSB-EP413416SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splA (splA) CSB-YP413417SUI
CSB-EP413417SUI
CSB-BP413417SUI
CSB-MP413417SUI
CSB-EP413417SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0754 membrane protein SaurJH1_1933 (SaurJH1_1933), partial CSB-YP413418SUI1
CSB-EP413418SUI1
CSB-BP413418SUI1
CSB-MP413418SUI1
CSB-EP413418SUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP413419SUI
CSB-EP413419SUI
CSB-BP413419SUI
CSB-MP413419SUI
CSB-EP413419SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP413420SUI
CSB-EP413420SUI
CSB-BP413420SUI
CSB-MP413420SUI
CSB-EP413420SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 10 kDa chaperonin (groS) CSB-YP413421SUI
CSB-EP413421SUI
CSB-BP413421SUI
CSB-MP413421SUI
CSB-EP413421SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP413422SUI
CSB-EP413422SUI
CSB-BP413422SUI
CSB-MP413422SUI
CSB-EP413422SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine-protein kinase rsbW (rsbW) CSB-YP413423SUI
CSB-EP413423SUI
CSB-BP413423SUI
CSB-MP413423SUI
CSB-EP413423SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L31 type B (rpmE2) CSB-YP413424SUI
CSB-EP413424SUI
CSB-BP413424SUI
CSB-MP413424SUI
CSB-EP413424SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoglucosamine mutase (glmM) CSB-YP413425SUI
CSB-EP413425SUI
CSB-BP413425SUI
CSB-MP413425SUI
CSB-EP413425SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S11 (rpsK) CSB-YP413426SUI
CSB-EP413426SUI
CSB-BP413426SUI
CSB-MP413426SUI
CSB-EP413426SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L6 (rplF) CSB-YP413427SUI
CSB-EP413427SUI
CSB-BP413427SUI
CSB-MP413427SUI
CSB-EP413427SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L24 (rplX) CSB-YP413428SUI
CSB-EP413428SUI
CSB-BP413428SUI
CSB-MP413428SUI
CSB-EP413428SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L16 (rplP) CSB-YP413429SUI
CSB-EP413429SUI
CSB-BP413429SUI
CSB-MP413429SUI
CSB-EP413429SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP413430SUI
CSB-EP413430SUI
CSB-BP413430SUI
CSB-MP413430SUI
CSB-EP413430SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease accessory protein UreF (ureF) CSB-YP413431SUI
CSB-EP413431SUI
CSB-BP413431SUI
CSB-MP413431SUI
CSB-EP413431SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP413432SUI
CSB-EP413432SUI
CSB-BP413432SUI
CSB-MP413432SUI
CSB-EP413432SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP413433SUI
CSB-EP413433SUI
CSB-BP413433SUI
CSB-MP413433SUI
CSB-EP413433SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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