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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio harveyi Sugar fermentation stimulation protein homolog (sfsA) CSB-YP415154VEA
CSB-EP415154VEA
CSB-BP415154VEA
CSB-MP415154VEA
CSB-EP415154VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Elongation factor P (efp) CSB-YP415155VEA
CSB-EP415155VEA
CSB-BP415155VEA
CSB-MP415155VEA
CSB-EP415155VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Histidine--tRNA ligase (hisS) CSB-YP415156VEA
CSB-EP415156VEA
CSB-BP415156VEA
CSB-MP415156VEA
CSB-EP415156VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi tRNA pseudouridine synthase B (truB) CSB-YP415157VEA
CSB-EP415157VEA
CSB-BP415157VEA
CSB-MP415157VEA
CSB-EP415157VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi DNA-binding protein fis (fis) CSB-YP415158VEA
CSB-EP415158VEA
CSB-BP415158VEA
CSB-MP415158VEA
CSB-EP415158VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L14 (rplN) CSB-YP415159VEA
CSB-EP415159VEA
CSB-BP415159VEA
CSB-MP415159VEA
CSB-EP415159VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Macrodomain Ter protein (matP) CSB-YP415160VEA
CSB-EP415160VEA
CSB-BP415160VEA
CSB-MP415160VEA
CSB-EP415160VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP415161VEA
CSB-EP415161VEA
CSB-BP415161VEA
CSB-MP415161VEA
CSB-EP415161VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi ATP synthase subunit delta 1 (atpH1) CSB-YP415162VEA
CSB-EP415162VEA
CSB-BP415162VEA
CSB-MP415162VEA
CSB-EP415162VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP415163VEA
CSB-EP415163VEA
CSB-BP415163VEA
CSB-MP415163VEA
CSB-EP415163VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP415164VEA
CSB-EP415164VEA
CSB-BP415164VEA
CSB-MP415164VEA
CSB-EP415164VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi DNA replication and repair protein recF (recF) CSB-YP415165VEA
CSB-EP415165VEA
CSB-BP415165VEA
CSB-MP415165VEA
CSB-EP415165VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP415166VEA
CSB-EP415166VEA
CSB-BP415166VEA
CSB-MP415166VEA
CSB-EP415166VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0178 protein VIBHAR_03247 (VIBHAR_03247) CSB-YP415167VEA
CSB-EP415167VEA
CSB-BP415167VEA
CSB-MP415167VEA
CSB-EP415167VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi ATP synthase subunit a 2 (atpB2), partial CSB-YP415168VEA1
CSB-EP415168VEA1
CSB-BP415168VEA1
CSB-MP415168VEA1
CSB-EP415168VEA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0502 protein VIBHAR_05349 (VIBHAR_05349) CSB-YP415169VEA
CSB-EP415169VEA
CSB-BP415169VEA
CSB-MP415169VEA
CSB-EP415169VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Dihydroorotase (pyrC) CSB-YP415170VEA
CSB-EP415170VEA
CSB-BP415170VEA
CSB-MP415170VEA
CSB-EP415170VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi ATP synthase subunit delta 2 (atpH2) CSB-YP415171VEA
CSB-EP415171VEA
CSB-BP415171VEA
CSB-MP415171VEA
CSB-EP415171VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Hydroxyethylthiazole kinase (thiM) CSB-YP415172VEA
CSB-EP415172VEA
CSB-BP415172VEA
CSB-MP415172VEA
CSB-EP415172VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Protein-export protein secB 1 (secB1) CSB-YP415173FDV
CSB-EP415173FDV
CSB-BP415173FDV
CSB-MP415173FDV
CSB-EP415173FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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