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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Aldo-keto reductase family 1 member C3 (AKR1C3) CSB-YP001544HU
CSB-BP001544HU
CSB-MP001544HU
CSB-EP001544HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (Pik3ca), partial CSB-YP017998MO
CSB-EP017998MO
CSB-BP017998MO
CSB-MP017998MO
CSB-EP017998MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Sarcolipin (SLN), partial CSB-YP021780RB
CSB-EP021780RB
CSB-BP021780RB
CSB-MP021780RB
CSB-EP021780RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken GTP-binding nuclear protein Ran (RAN) CSB-YP019307CH
CSB-EP019307CH
CSB-BP019307CH
CSB-MP019307CH
CSB-EP019307CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Epidermal growth factor receptor substrate 15 (EPS15), partial CSB-YP007748HU
CSB-BP007748HU
CSB-MP007748HU
CSB-EP007748HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein AF-9 (MLLT3) CSB-YP014635HU
CSB-EP014635HU
CSB-BP014635HU
CSB-EP014635HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NK1 transcription factor-related protein 2 (Nkx1-2) CSB-YP020723MO
CSB-EP020723MO
CSB-BP020723MO
CSB-MP020723MO
CSB-EP020723MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Homeobox protein HMX3 (Hmx3) CSB-YP010588MO
CSB-EP010588MO
CSB-BP010588MO
CSB-MP010588MO
CSB-EP010588MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Homeobox protein Nkx-2.2 (Nkx2-2) CSB-YP015842MO
CSB-BP015842MO
CSB-MP015842MO
CSB-EP015842MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Dipeptidyl aminopeptidase-like protein 6 (DPP6), partial CSB-YP007140BO
CSB-EP007140BO
CSB-BP007140BO
CSB-MP007140BO
CSB-EP007140BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Neurolysin, mitochondrial (NLN), partial CSB-YP015860RB
CSB-EP015860RB
CSB-BP015860RB
CSB-MP015860RB
CSB-EP015860RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tyrosine-protein kinase Tec (TEC) CSB-YP023367HU
CSB-EP023367HU
CSB-BP023367HU
CSB-MP023367HU
CSB-EP023367HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Proto-oncogene tyrosine-protein kinase LCK (LCK), partial CSB-YP012806CH
CSB-EP012806CH
CSB-BP012806CH
CSB-MP012806CH
CSB-EP012806CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tyrosine-protein kinase FRK (FRK) CSB-YP008988HU
CSB-EP008988HU
CSB-BP008988HU
CSB-MP008988HU
CSB-EP008988HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Condensin-2 complex subunit D3 (NCAPD3), partial CSB-YP015515HU
CSB-EP015515HU
CSB-BP015515HU
CSB-MP015515HU
CSB-EP015515HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Leukemia inhibitory factor receptor (LIFR), partial CSB-YP012929HU
CSB-EP012929HU
CSB-BP012929HU
CSB-EP012929HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Leukemia inhibitory factor receptor (Lifr), partial CSB-YP012929MO
CSB-EP012929MO
CSB-BP012929MO
CSB-MP012929MO
CSB-EP012929MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Thrombopoietin (THPO) CSB-YP023509DO
CSB-EP023509DO
CSB-BP023509DO
CSB-MP023509DO
CSB-EP023509DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cyclin-dependent kinase 4 inhibitor B (CDKN2B) CSB-YP005092HU
CSB-BP005092HU
CSB-MP005092HU
CSB-EP005092HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Lysosomal Pro-X carboxypeptidase (PRCP) CSB-YP018636HU
CSB-EP018636HU
CSB-BP018636HU
CSB-MP018636HU
CSB-EP018636HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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