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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rat Cofilin-1 (Cfl1) CSB-YP005280RA
CSB-EP005280RA
CSB-BP005280RA
CSB-MP005280RA
CSB-EP005280RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) CSB-YP013383RB
CSB-EP013383RB
CSB-BP013383RB
CSB-MP013383RB
CSB-EP013383RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Protein-lysine 6-oxidase (LOX) CSB-YP013038PI
CSB-EP013038PI
CSB-BP013038PI
CSB-MP013038PI
CSB-EP013038PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Peptidyl-prolyl cis-trans isomerase C (PPIC) CSB-YP018473HU
CSB-EP018473HU
CSB-BP018473HU
CSB-MP018473HU
CSB-EP018473HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Voltage-dependent anion-selective channel protein 1 (VDAC1), partial CSB-YP025821BO
CSB-EP025821BO
CSB-BP025821BO
CSB-MP025821BO
CSB-EP025821BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (Acadm) CSB-YP001126MO
CSB-EP001126MO
CSB-BP001126MO
CSB-MP001126MO
CSB-EP001126MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (ACADSB) CSB-YP001128HU
CSB-EP001128HU
CSB-BP001128HU
CSB-MP001128HU
CSB-EP001128HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Chromobox protein homolog 5 (CBX5) CSB-YP004601HU
CSB-BP004601HU
CSB-MP004601HU
CSB-EP004601HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mitogen-activated protein kinase 9 (MAPK9) CSB-YP013471HU
CSB-BP013471HU
CSB-MP013471HU
CSB-EP013471HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Platelet-activating factor receptor (Ptafr), partial CSB-YP018946RA1
CSB-EP018946RA1
CSB-BP018946RA1
CSB-MP018946RA1
CSB-EP018946RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phosphorylase b kinase regulatory subunit alpha, liver isoform (PHKA2), partial CSB-YP017923HU
CSB-EP017923HU
CSB-BP017923HU
CSB-MP017923HU
CSB-EP017923HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ran GTPase-activating protein 1 (RANGAP1) CSB-YP019318HU
CSB-EP019318HU
CSB-BP019318HU
CSB-MP019318HU
CSB-EP019318HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Signal-induced proliferation-associated protein 1 (Sipa1), partial CSB-YP021330MO
CSB-EP021330MO
CSB-BP021330MO
CSB-MP021330MO
CSB-EP021330MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat G-protein coupled receptor 1 (Gpr1), partial CSB-YP009728RA1
CSB-EP009728RA1
CSB-BP009728RA1
CSB-MP009728RA1
CSB-EP009728RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human C-C chemokine receptor type 10 (CCR10), partial CSB-YP004840HU1
CSB-EP004840HU1
CSB-BP004840HU1
CSB-MP004840HU1
CSB-EP004840HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Chemokine XC receptor 1 (XCR1), partial CSB-YP026188HU1
CSB-EP026188HU1
CSB-BP026188HU1
CSB-MP026188HU1
CSB-EP026188HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Synaptotagmin-2 (Syt2), partial CSB-YP023038MO1
CSB-EP023038MO1
CSB-BP023038MO1
CSB-MP023038MO1
CSB-EP023038MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Krueppel-like factor 1 (Klf1) CSB-YP012383MO
CSB-EP012383MO
CSB-BP012383MO
CSB-MP012383MO
CSB-EP012383MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Crk-like protein (CRKL) CSB-YP005980HU
CSB-EP005980HU
CSB-BP005980HU
CSB-MP005980HU
CSB-EP005980HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Transcription factor GATA-6 (Gata6) CSB-YP009279RA
CSB-EP009279RA
CSB-BP009279RA
CSB-MP009279RA
CSB-EP009279RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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