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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Human Bone morphogenetic protein 8B (BMP8B) CSB-YP002746HU
CSB-EP002746HU
CSB-BP002746HU
CSB-MP002746HU
CSB-EP002746HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Bifunctional Epoxide hydrolase 2 (EPHX2) CSB-YP007735HU
CSB-BP007735HU
CSB-MP007735HU
CSB-EP007735HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (NDUFA9) CSB-YP015634BO
CSB-EP015634BO
CSB-BP015634BO
CSB-MP015634BO
CSB-EP015634BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Neuromedin-U-25 (NMU) CSB-YP015902PI
CSB-EP015902PI
CSB-BP015902PI
CSB-MP015902PI
CSB-EP015902PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Thyrotropin-releasing hormone receptor (TRHR), partial CSB-YP024441HU1
CSB-EP024441HU1
CSB-BP024441HU1
CSB-MP024441HU1
CSB-EP024441HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Prostaglandin E2 receptor EP1 subtype (PTGER1), partial CSB-YP018970HU1
CSB-EP018970HU1
CSB-BP018970HU1
CSB-MP018970HU1
CSB-EP018970HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Vasoactive intestinal polypeptide receptor 2 (Vipr2), partial CSB-YP025860RA1
CSB-EP025860RA1
CSB-BP025860RA1
CSB-MP025860RA1
CSB-EP025860RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Glypican-1 (Gpc1) CSB-YP009703RA
CSB-EP009703RA
CSB-BP009703RA
CSB-MP009703RA
CSB-EP009703RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Carbonic anhydrase 5A, mitochondrial (CA5A) CSB-YP004373HU
CSB-EP004373HU
CSB-BP004373HU
CSB-EP004373HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Nitric oxide synthase, inducible (NOS2), partial CSB-YP015943HU
CSB-BP015943HU
CSB-MP015943HU
CSB-EP015943HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tyrosine-protein phosphatase non-receptor type 2 (Ptpn2) CSB-YP019031RA
CSB-EP019031RA
CSB-BP019031RA
CSB-MP019031RA
CSB-EP019031RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Merlin (NF2) CSB-YP015741HU
CSB-BP015741HU
CSB-MP015741HU
CSB-EP015741HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sepiapterin reductase (SPR) CSB-YP022605HU
CSB-BP022605HU
CSB-MP022605HU
CSB-EP022605HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ras-related protein Rab-12 (Rab12) CSB-YP019160MO
CSB-EP019160MO
CSB-BP019160MO
CSB-MP019160MO
CSB-EP019160MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ras-related protein Rab-24 (Rab24) CSB-YP019174MO
CSB-EP019174MO
CSB-BP019174MO
CSB-MP019174MO
CSB-EP019174MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ras-related protein Rab-20 (Rab20) CSB-YP019170MO
CSB-EP019170MO
CSB-BP019170MO
CSB-MP019170MO
CSB-EP019170MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Adenosine receptor A3 (ADORA3), partial CSB-YP001379SH1
CSB-EP001379SH1
CSB-BP001379SH1
CSB-MP001379SH1
CSB-EP001379SH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Corticotropin-releasing factor receptor 1 (Crhr1), partial CSB-YP005965MO1
CSB-EP005965MO1
CSB-BP005965MO1
CSB-MP005965MO1
CSB-EP005965MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta (Rhesus macaque) Platelet-activating factor receptor (PTAFR), partial CSB-YP018946MOW1
CSB-EP018946MOW1
CSB-BP018946MOW1
CSB-MP018946MOW1
CSB-EP018946MOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Bombesin receptor subtype-3 (BRS3), partial CSB-YP002820GU1
CSB-EP002820GU1
CSB-BP002820GU1
CSB-MP002820GU1
CSB-EP002820GU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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