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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus pumilus UPF0223 protein BPUM_1362 (BPUM_1362) CSB-YP424438BOL
CSB-EP424438BOL
CSB-BP424438BOL
CSB-MP424438BOL
CSB-EP424438BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Phosphopantetheine adenylyltransferase (coaD) CSB-YP424439BOL
CSB-EP424439BOL
CSB-BP424439BOL
CSB-MP424439BOL
CSB-EP424439BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP424440BOL
CSB-EP424440BOL
CSB-BP424440BOL
CSB-MP424440BOL
CSB-EP424440BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Dihydroorotase (pyrC) CSB-YP424441BOL
CSB-EP424441BOL
CSB-BP424441BOL
CSB-MP424441BOL
CSB-EP424441BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0296 protein BPUM_1466 (BPUM_1466) CSB-YP424442BOL
CSB-EP424442BOL
CSB-BP424442BOL
CSB-MP424442BOL
CSB-EP424442BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Transcription factor fapR (fapR) CSB-YP424443BOL
CSB-EP424443BOL
CSB-BP424443BOL
CSB-MP424443BOL
CSB-EP424443BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L19 (rplS) CSB-YP424444BOL
CSB-EP424444BOL
CSB-BP424444BOL
CSB-MP424444BOL
CSB-EP424444BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus ATP-dependent protease subunit ClpQ (clpQ) CSB-YP424445BOL
CSB-EP424445BOL
CSB-BP424445BOL
CSB-MP424445BOL
CSB-EP424445BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 30S ribosomal protein S15 (rpsO) CSB-YP424446BOL
CSB-EP424446BOL
CSB-BP424446BOL
CSB-MP424446BOL
CSB-EP424446BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (BPUM_1604) CSB-YP424447BOL
CSB-EP424447BOL
CSB-BP424447BOL
CSB-MP424447BOL
CSB-EP424447BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Lactate utilization protein B (lutB) CSB-YP424448BOL
CSB-EP424448BOL
CSB-BP424448BOL
CSB-MP424448BOL
CSB-EP424448BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Small, acid-soluble spore protein P (sspP) CSB-YP424449BOL
CSB-EP424449BOL
CSB-BP424449BOL
CSB-MP424449BOL
CSB-EP424449BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP424450BOL
CSB-EP424450BOL
CSB-BP424450BOL
CSB-MP424450BOL
CSB-EP424450BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Holliday junction resolvase recU (recU) CSB-YP424451BOL
CSB-EP424451BOL
CSB-BP424451BOL
CSB-MP424451BOL
CSB-EP424451BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0302 protein BPUM_1989 (BPUM_1989) CSB-YP424452BOL
CSB-EP424452BOL
CSB-BP424452BOL
CSB-MP424452BOL
CSB-EP424452BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Indole-3-glycerol phosphate synthase (trpC) CSB-YP424453BOL
CSB-EP424453BOL
CSB-BP424453BOL
CSB-MP424453BOL
CSB-EP424453BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP424454BOL
CSB-EP424454BOL
CSB-BP424454BOL
CSB-MP424454BOL
CSB-EP424454BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Phosphopentomutase (deoB) CSB-YP424455BOL
CSB-EP424455BOL
CSB-BP424455BOL
CSB-MP424455BOL
CSB-EP424455BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 3-dehydroquinate dehydratase (aroQ) CSB-YP424456BOL
CSB-EP424456BOL
CSB-BP424456BOL
CSB-MP424456BOL
CSB-EP424456BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP424457BOL
CSB-EP424457BOL
CSB-BP424457BOL
CSB-MP424457BOL
CSB-EP424457BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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