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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rickettsia massiliae 30S ribosomal protein S3 (rpsC) CSB-YP424398RIN
CSB-EP424398RIN
CSB-BP424398RIN
CSB-MP424398RIN
CSB-EP424398RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP424399RIN
CSB-EP424399RIN
CSB-BP424399RIN
CSB-MP424399RIN
CSB-EP424399RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae tRNA modification GTPase MnmE (mnmE) CSB-YP424400RIN
CSB-EP424400RIN
CSB-BP424400RIN
CSB-MP424400RIN
CSB-EP424400RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae GTPase obg (obg) CSB-YP424401RIN
CSB-EP424401RIN
CSB-BP424401RIN
CSB-MP424401RIN
CSB-EP424401RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP424402RIN
CSB-EP424402RIN
CSB-BP424402RIN
CSB-MP424402RIN
CSB-EP424402RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Proline--tRNA ligase (proS), partial CSB-YP424403TKE
CSB-EP424403TKE
CSB-BP424403TKE
CSB-MP424403TKE
CSB-EP424403TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP424404TKE
CSB-EP424404TKE
CSB-BP424404TKE
CSB-MP424404TKE
CSB-EP424404TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Acylphosphatase (acyP) CSB-YP424405TKE
CSB-EP424405TKE
CSB-BP424405TKE
CSB-MP424405TKE
CSB-EP424405TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Lysine--tRNA ligase (lysS) CSB-YP424406TKE
CSB-EP424406TKE
CSB-BP424406TKE
CSB-MP424406TKE
CSB-EP424406TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 30S ribosomal protein S7 (rpsG) CSB-YP424407TKE
CSB-EP424407TKE
CSB-BP424407TKE
CSB-MP424407TKE
CSB-EP424407TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 30S ribosomal protein S19 (rpsS) CSB-YP424408TKE
CSB-EP424408TKE
CSB-BP424408TKE
CSB-MP424408TKE
CSB-EP424408TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 50S ribosomal protein L16 (rplP) CSB-YP424409TKE
CSB-EP424409TKE
CSB-BP424409TKE
CSB-MP424409TKE
CSB-EP424409TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 50S ribosomal protein L6 (rplF) CSB-YP424410TKE
CSB-EP424410TKE
CSB-BP424410TKE
CSB-MP424410TKE
CSB-EP424410TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 50S ribosomal protein L30 (rpmD) CSB-YP424411TKE
CSB-EP424411TKE
CSB-BP424411TKE
CSB-MP424411TKE
CSB-EP424411TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 30S ribosomal protein S11 (rpsK) CSB-YP424412TKE
CSB-EP424412TKE
CSB-BP424412TKE
CSB-MP424412TKE
CSB-EP424412TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP424413TKE
CSB-EP424413TKE
CSB-BP424413TKE
CSB-MP424413TKE
CSB-EP424413TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae DNA mismatch repair protein mutL (mutL), partial CSB-YP424414TKE
CSB-EP424414TKE
CSB-BP424414TKE
CSB-MP424414TKE
CSB-EP424414TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Protein hfq (hfq) CSB-YP424415TKE
CSB-EP424415TKE
CSB-BP424415TKE
CSB-MP424415TKE
CSB-EP424415TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP424416TKE
CSB-EP424416TKE
CSB-BP424416TKE
CSB-MP424416TKE
CSB-EP424416TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Probable 2-phosphosulfolactate phosphatase (comB) CSB-YP424417TKE
CSB-EP424417TKE
CSB-BP424417TKE
CSB-MP424417TKE
CSB-EP424417TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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