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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Citrobacter koseri Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP425279CWS
CSB-EP425279CWS
CSB-BP425279CWS
CSB-MP425279CWS
CSB-EP425279CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri CDP-diacylglycerol pyrophosphatase (cdh), partial CSB-YP425280CWS
CSB-EP425280CWS
CSB-BP425280CWS
CSB-MP425280CWS
CSB-EP425280CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri L-rhamnose mutarotase (rhaM) CSB-YP425281CWS
CSB-EP425281CWS
CSB-BP425281CWS
CSB-MP425281CWS
CSB-EP425281CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Lipid-A-disaccharide synthase (lpxB) CSB-YP425282CWS
CSB-EP425282CWS
CSB-BP425282CWS
CSB-MP425282CWS
CSB-EP425282CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri [Protein-PII] uridylyltransferase (glnD), partial CSB-YP425283CWS
CSB-EP425283CWS
CSB-BP425283CWS
CSB-MP425283CWS
CSB-EP425283CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Aspartate 1-decarboxylase (panD) CSB-YP425284CWS
CSB-EP425284CWS
CSB-BP425284CWS
CSB-MP425284CWS
CSB-EP425284CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Protein translocase subunit SecA (secA), partial CSB-YP425285CWS
CSB-EP425285CWS
CSB-BP425285CWS
CSB-MP425285CWS
CSB-EP425285CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribulokinase (araB), partial CSB-YP425286CWS
CSB-BP425286CWS
CSB-MP425286CWS
CSB-EP425286CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP425287CWS
CSB-EP425287CWS
CSB-BP425287CWS
CSB-MP425287CWS
CSB-EP425287CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Dihydrodipicolinate reductase (dapB) CSB-YP425288CWS
CSB-EP425288CWS
CSB-BP425288CWS
CSB-MP425288CWS
CSB-EP425288CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Homoserine kinase (thrB) CSB-YP425289CWS
CSB-EP425289CWS
CSB-BP425289CWS
CSB-MP425289CWS
CSB-EP425289CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UPF0442 protein CKO_03436 (CKO_03436), partial CSB-YP425290CWS1
CSB-EP425290CWS1
CSB-BP425290CWS1
CSB-MP425290CWS1
CSB-EP425290CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP425291CWS
CSB-EP425291CWS
CSB-BP425291CWS
CSB-MP425291CWS
CSB-EP425291CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Adenylosuccinate synthetase (purA) CSB-YP425292CWS
CSB-EP425292CWS
CSB-BP425292CWS
CSB-MP425292CWS
CSB-EP425292CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Fumarate reductase subunit C (frdC), partial CSB-YP425293CWS1
CSB-EP425293CWS1
CSB-BP425293CWS1
CSB-MP425293CWS1
CSB-EP425293CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri LexA repressor (lexA) CSB-YP425294CWS
CSB-EP425294CWS
CSB-BP425294CWS
CSB-MP425294CWS
CSB-EP425294CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 30S ribosomal protein S16 (rpsP) CSB-YP425295CWS
CSB-EP425295CWS
CSB-BP425295CWS
CSB-MP425295CWS
CSB-EP425295CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Carbon storage regulator homolog (csrA) CSB-YP425296CWS
CSB-EP425296CWS
CSB-BP425296CWS
CSB-MP425296CWS
CSB-EP425296CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 5'/3'-nucleotidase SurE (surE) CSB-YP425297CWS
CSB-EP425297CWS
CSB-BP425297CWS
CSB-MP425297CWS
CSB-EP425297CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP425298CWS
CSB-EP425298CWS
CSB-BP425298CWS
CSB-MP425298CWS
CSB-EP425298CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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