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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O139:H28 Altronate oxidoreductase (uxaB) CSB-YP425079EJD
CSB-EP425079EJD
CSB-BP425079EJD
CSB-MP425079EJD
CSB-EP425079EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Spermidine export protein MdtI (mdtI), partial CSB-YP425080EJD1
CSB-EP425080EJD1
CSB-BP425080EJD1
CSB-MP425080EJD1
CSB-EP425080EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Electron transport complex protein RnfB (rnfB) CSB-YP425081EJD
CSB-EP425081EJD
CSB-BP425081EJD
CSB-MP425081EJD
CSB-EP425081EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ribonuclease T (rnt) CSB-YP425082EJD
CSB-EP425082EJD
CSB-BP425082EJD
CSB-MP425082EJD
CSB-EP425082EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Putative phosphotransferase ydiA (ydiA) CSB-YP425083EJD
CSB-EP425083EJD
CSB-BP425083EJD
CSB-MP425083EJD
CSB-EP425083EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Cell division activator CedA (cedA) CSB-YP425084EJD
CSB-EP425084EJD
CSB-BP425084EJD
CSB-MP425084EJD
CSB-EP425084EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Arginine N-succinyltransferase (astA) CSB-YP425085EJD
CSB-EP425085EJD
CSB-BP425085EJD
CSB-MP425085EJD
CSB-EP425085EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP425086EJD
CSB-EP425086EJD
CSB-BP425086EJD
CSB-MP425086EJD
CSB-EP425086EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP425087EJD
CSB-EP425087EJD
CSB-BP425087EJD
CSB-MP425087EJD
CSB-EP425087EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (otsA) CSB-YP425088EJD
CSB-EP425088EJD
CSB-BP425088EJD
CSB-MP425088EJD
CSB-EP425088EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP425089EJD1
CSB-EP425089EJD1
CSB-BP425089EJD1
CSB-MP425089EJD1
CSB-EP425089EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP425090EJD
CSB-EP425090EJD
CSB-BP425090EJD
CSB-MP425090EJD
CSB-EP425090EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Hydroxyethylthiazole kinase (thiM) CSB-YP425091EJD
CSB-EP425091EJD
CSB-BP425091EJD
CSB-MP425091EJD
CSB-EP425091EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 S-formylglutathione hydrolase yeiG (yeiG) CSB-YP425092EJD
CSB-EP425092EJD
CSB-BP425092EJD
CSB-MP425092EJD
CSB-EP425092EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Periplasmic nitrate reductase (napA) CSB-YP425093EJD
CSB-EP425093EJD
CSB-BP425093EJD
CSB-MP425093EJD
CSB-EP425093EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP425094EJD1
CSB-EP425094EJD1
CSB-BP425094EJD1
CSB-MP425094EJD1
CSB-EP425094EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD) CSB-YP425095EJD
CSB-EP425095EJD
CSB-BP425095EJD
CSB-MP425095EJD
CSB-EP425095EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP425096EJD1
CSB-EP425096EJD1
CSB-BP425096EJD1
CSB-MP425096EJD1
CSB-EP425096EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Penicillin-insensitive murein endopeptidase (mepA) CSB-YP425097EJD
CSB-EP425097EJD
CSB-BP425097EJD
CSB-MP425097EJD
CSB-EP425097EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Putative ion-transport protein yfeO (yfeO), partial CSB-YP425098EJD
CSB-EP425098EJD
CSB-BP425098EJD
CSB-MP425098EJD
CSB-EP425098EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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