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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rickettsia canadensis tRNA pseudouridine synthase B (truB) CSB-YP425399RMR
CSB-EP425399RMR
CSB-BP425399RMR
CSB-MP425399RMR
CSB-EP425399RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Recombination protein RecR (recR) CSB-YP425400RMR
CSB-EP425400RMR
CSB-BP425400RMR
CSB-MP425400RMR
CSB-EP425400RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP425401RMR
CSB-EP425401RMR
CSB-BP425401RMR
CSB-MP425401RMR
CSB-EP425401RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 30S ribosomal protein S4 (rpsD) CSB-YP425402RMR
CSB-EP425402RMR
CSB-BP425402RMR
CSB-MP425402RMR
CSB-EP425402RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis ATP-dependent protease subunit HslV (hslV) CSB-YP425403RMR
CSB-EP425403RMR
CSB-BP425403RMR
CSB-MP425403RMR
CSB-EP425403RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis DNA mismatch repair protein MutS (mutS), partial CSB-YP425404RMR
CSB-EP425404RMR
CSB-BP425404RMR
CSB-MP425404RMR
CSB-EP425404RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Adenylate kinase (adk) CSB-YP425405RMR
CSB-EP425405RMR
CSB-BP425405RMR
CSB-MP425405RMR
CSB-EP425405RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 50S ribosomal protein L5 (rplE) CSB-YP425406RMR
CSB-EP425406RMR
CSB-BP425406RMR
CSB-MP425406RMR
CSB-EP425406RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 50S ribosomal protein L29 (rpmC) CSB-YP425407RMR
CSB-EP425407RMR
CSB-BP425407RMR
CSB-MP425407RMR
CSB-EP425407RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP425408RMR
CSB-EP425408RMR
CSB-BP425408RMR
CSB-MP425408RMR
CSB-EP425408RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP425409RMR
CSB-EP425409RMR
CSB-BP425409RMR
CSB-MP425409RMR
CSB-EP425409RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis ATP synthase gamma chain (atpG) CSB-YP425410RMR
CSB-EP425410RMR
CSB-BP425410RMR
CSB-MP425410RMR
CSB-EP425410RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Tyrosine recombinase XerC (xerC) CSB-YP425411RMR
CSB-EP425411RMR
CSB-BP425411RMR
CSB-MP425411RMR
CSB-EP425411RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP425412RMR
CSB-EP425412RMR
CSB-BP425412RMR
CSB-MP425412RMR
CSB-EP425412RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 30S ribosomal protein S16 (rpsP) CSB-YP425413RMR
CSB-EP425413RMR
CSB-BP425413RMR
CSB-MP425413RMR
CSB-EP425413RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP425414RIN
CSB-EP425414RIN
CSB-BP425414RIN
CSB-MP425414RIN
CSB-EP425414RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae tRNA (Ile)-lysidine synthase CSB-YP425415RIN
CSB-EP425415RIN
CSB-BP425415RIN
CSB-MP425415RIN
CSB-EP425415RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 30S ribosomal protein S2 (rpsB) CSB-YP425416RIN
CSB-EP425416RIN
CSB-BP425416RIN
CSB-MP425416RIN
CSB-EP425416RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae NADH-quinone oxidoreductase subunit F (nuoF) CSB-YP425417RIN
CSB-EP425417RIN
CSB-BP425417RIN
CSB-MP425417RIN
CSB-EP425417RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 50S ribosomal protein L11 (rplK) CSB-YP425418RIN
CSB-EP425418RIN
CSB-BP425418RIN
CSB-MP425418RIN
CSB-EP425418RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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