Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Caldivirga maquilingensis Translation initiation factor 5A (eIF5A) CSB-YP428100CVY
CSB-EP428100CVY
CSB-BP428100CVY
CSB-MP428100CVY
CSB-EP428100CVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldivirga maquilingensis 30S ribosomal protein S4P (rps4p) CSB-YP428101CVY
CSB-EP428101CVY
CSB-BP428101CVY
CSB-MP428101CVY
CSB-EP428101CVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldivirga maquilingensis Protein Cmaq_1209 (Cmaq_1209) CSB-YP428102CVY
CSB-EP428102CVY
CSB-BP428102CVY
CSB-MP428102CVY
CSB-EP428102CVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Phosphomethylpyrimidine synthase (thiC) CSB-YP428103AUQ
CSB-EP428103AUQ
CSB-BP428103AUQ
CSB-MP428103AUQ
CSB-EP428103AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 30S ribosomal protein S20 (rpsT) CSB-YP428104AUQ
CSB-EP428104AUQ
CSB-BP428104AUQ
CSB-MP428104AUQ
CSB-EP428104AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Protein hfq (hfq) CSB-YP428105AUQ
CSB-EP428105AUQ
CSB-BP428105AUQ
CSB-MP428105AUQ
CSB-EP428105AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP428106AUQ
CSB-EP428106AUQ
CSB-BP428106AUQ
CSB-MP428106AUQ
CSB-EP428106AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Enolase (eno) CSB-YP428107AUQ
CSB-EP428107AUQ
CSB-BP428107AUQ
CSB-MP428107AUQ
CSB-EP428107AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii UPF0365 protein Clos_1242 (Clos_1242), partial CSB-YP428108AUQ1
CSB-EP428108AUQ1
CSB-BP428108AUQ1
CSB-MP428108AUQ1
CSB-EP428108AUQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 3-dehydroquinate synthase (aroB) CSB-YP428109AUQ
CSB-EP428109AUQ
CSB-BP428109AUQ
CSB-MP428109AUQ
CSB-EP428109AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP428110AUQ
CSB-EP428110AUQ
CSB-BP428110AUQ
CSB-MP428110AUQ
CSB-EP428110AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 50S ribosomal protein L19 (rplS) CSB-YP428111AUQ
CSB-EP428111AUQ
CSB-BP428111AUQ
CSB-MP428111AUQ
CSB-EP428111AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii L-seryl-tRNA (Sec) selenium transferase CSB-YP428112AUQ
CSB-EP428112AUQ
CSB-BP428112AUQ
CSB-MP428112AUQ
CSB-EP428112AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 50S ribosomal protein L21 (rplU) CSB-YP428113AUQ
CSB-EP428113AUQ
CSB-BP428113AUQ
CSB-MP428113AUQ
CSB-EP428113AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii tRNA (guanine-N (7)-)-methyltransferase CSB-YP428114AUQ
CSB-EP428114AUQ
CSB-BP428114AUQ
CSB-MP428114AUQ
CSB-EP428114AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP428115AUQ
CSB-EP428115AUQ
CSB-BP428115AUQ
CSB-MP428115AUQ
CSB-EP428115AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Glucosamine-6-phosphate deaminase (nagB) CSB-YP428116AUQ
CSB-EP428116AUQ
CSB-BP428116AUQ
CSB-MP428116AUQ
CSB-EP428116AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP428117AUQ
CSB-EP428117AUQ
CSB-BP428117AUQ
CSB-MP428117AUQ
CSB-EP428117AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii ATP synthase subunit c (atpE), partial CSB-YP428118AUQ1
CSB-EP428118AUQ1
CSB-BP428118AUQ1
CSB-MP428118AUQ1
CSB-EP428118AUQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Bifunctional protein GlmU (glmU) CSB-YP428119AUQ
CSB-EP428119AUQ
CSB-BP428119AUQ
CSB-MP428119AUQ
CSB-EP428119AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>