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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Putative ankyrin repeat domain-containing protein ENSP00000383090 CSB-YP428500HU
CSB-EP428500HU
CSB-BP428500HU
CSB-MP428500HU
CSB-EP428500HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM25D/E (FAM25D) CSB-YP428501HU
CSB-EP428501HU
CSB-BP428501HU
CSB-MP428501HU
CSB-EP428501HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Maintenance of mitochondrial morphology protein 1 (MMM1), partial CSB-YP428502DWQ
CSB-EP428502DWQ
CSB-BP428502DWQ
CSB-MP428502DWQ
CSB-EP428502DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Amino-acid acetyltransferase, mitochondrial (ARG2), partial CSB-YP428503DWQ
CSB-EP428503DWQ
CSB-BP428503DWQ
CSB-MP428503DWQ
CSB-EP428503DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Protein SEY1 (SEY1), partial CSB-YP428504DWQ1
CSB-EP428504DWQ1
CSB-BP428504DWQ1
CSB-MP428504DWQ1
CSB-EP428504DWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Glutamyl-tRNA (Gln) amidotransferase subunit A, mitochondrial, partial CSB-YP428505DWQ
CSB-EP428505DWQ
CSB-BP428505DWQ
CSB-MP428505DWQ
CSB-EP428505DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (CC1G_08401), partial CSB-YP428506DWQ
CSB-EP428506DWQ
CSB-BP428506DWQ
CSB-MP428506DWQ
CSB-EP428506DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP428507DWQ
CSB-EP428507DWQ
CSB-BP428507DWQ
CSB-MP428507DWQ
CSB-EP428507DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Trehalose-phosphatase (Bm1_08695) CSB-YP428508BWV
CSB-EP428508BWV
CSB-BP428508BWV
CSB-MP428508BWV
CSB-EP428508BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea 40S ribosomal protein S1 (RPS1) CSB-YP428509DWQ
CSB-EP428509DWQ
CSB-BP428509DWQ
CSB-MP428509DWQ
CSB-EP428509DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Elongation factor G, mitochondrial (MEF1), partial CSB-YP428510DWQ
CSB-EP428510DWQ
CSB-BP428510DWQ
CSB-MP428510DWQ
CSB-EP428510DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi UPF0518 protein Bm1_18400 (Bm1_18400), partial CSB-YP428511BWV
CSB-EP428511BWV
CSB-BP428511BWV
CSB-MP428511BWV
CSB-EP428511BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Vacuolar protein sorting/targeting protein 10 (VPS10), partial CSB-YP428512DWQ
CSB-EP428512DWQ
CSB-BP428512DWQ
CSB-MP428512DWQ
CSB-EP428512DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa Maintenance of mitochondrial morphology protein 1 (MMM1), partial CSB-YP428513MNH
CSB-EP428513MNH
CSB-BP428513MNH
CSB-MP428513MNH
CSB-EP428513MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa Protein GET1 (GET1), partial CSB-YP428514MNH1
CSB-EP428514MNH1
CSB-BP428514MNH1
CSB-MP428514MNH1
CSB-EP428514MNH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa 3-ketoacyl-CoA reductase (MGL_1839), partial CSB-YP428515MNH
CSB-EP428515MNH
CSB-BP428515MNH
CSB-MP428515MNH
CSB-EP428515MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Ribosome biogenesis protein WDR12 homolog (Bm1_47965) CSB-YP428516BWV
CSB-EP428516BWV
CSB-BP428516BWV
CSB-MP428516BWV
CSB-EP428516BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Elongation factor Ts, mitochondrial (Bm1_50845) CSB-YP428517BWV
CSB-EP428517BWV
CSB-BP428517BWV
CSB-MP428517BWV
CSB-EP428517BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Austrelaps superbus Short neurotoxin 1 CSB-YP428518DPN
CSB-EP428518DPN
CSB-BP428518DPN
CSB-MP428518DPN
CSB-EP428518DPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum ATP synthase subunit alpha, chloroplastic (atpA), partial CSB-YP428519CWK
CSB-EP428519CWK
CSB-BP428519CWK
CSB-MP428519CWK
CSB-EP428519CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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