Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Delftia acidovorans 30S ribosomal protein S13 (rpsM) CSB-YP429100DHV
CSB-EP429100DHV
CSB-BP429100DHV
CSB-MP429100DHV
CSB-EP429100DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP429101DHV
CSB-EP429101DHV
CSB-BP429101DHV
CSB-MP429101DHV
CSB-EP429101DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Urease subunit gamma (ureA) CSB-YP429102DHV
CSB-EP429102DHV
CSB-BP429102DHV
CSB-MP429102DHV
CSB-EP429102DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans ATP-dependent protease subunit HslV (hslV) CSB-YP429103DHV
CSB-EP429103DHV
CSB-BP429103DHV
CSB-MP429103DHV
CSB-EP429103DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP429104DHV
CSB-EP429104DHV
CSB-BP429104DHV
CSB-MP429104DHV
CSB-EP429104DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Aspartate 1-decarboxylase (panD) CSB-YP429105DHV
CSB-EP429105DHV
CSB-BP429105DHV
CSB-MP429105DHV
CSB-EP429105DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Elongation factor P (efp) CSB-YP429106DHV
CSB-EP429106DHV
CSB-BP429106DHV
CSB-MP429106DHV
CSB-EP429106DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Bifunctional protein FolD (folD) CSB-YP429107DHV
CSB-EP429107DHV
CSB-BP429107DHV
CSB-MP429107DHV
CSB-EP429107DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP429108DHV
CSB-EP429108DHV
CSB-BP429108DHV
CSB-MP429108DHV
CSB-EP429108DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 60 kDa chaperonin (groL), partial CSB-YP429109DHV
CSB-EP429109DHV
CSB-BP429109DHV
CSB-MP429109DHV
CSB-EP429109DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP429110DHV
CSB-EP429110DHV
CSB-BP429110DHV
CSB-MP429110DHV
CSB-EP429110DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Cysteine--tRNA ligase (cysS) CSB-YP429111DHV
CSB-EP429111DHV
CSB-BP429111DHV
CSB-MP429111DHV
CSB-EP429111DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 30S ribosomal protein S9 (rpsI) CSB-YP429112DHV
CSB-EP429112DHV
CSB-BP429112DHV
CSB-MP429112DHV
CSB-EP429112DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Biotin synthase (bioB) CSB-YP429113DHV
CSB-EP429113DHV
CSB-BP429113DHV
CSB-MP429113DHV
CSB-EP429113DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP429114DHV
CSB-EP429114DHV
CSB-BP429114DHV
CSB-MP429114DHV
CSB-EP429114DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens FMN reductase (NADH) RutF CSB-YP429115AYS
CSB-EP429115AYS
CSB-BP429115AYS
CSB-MP429115AYS
CSB-EP429115AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP429116AYS
CSB-EP429116AYS
CSB-BP429116AYS
CSB-MP429116AYS
CSB-EP429116AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Mannosylfructose-phosphate phosphatase (mfppA) CSB-YP429117AYS
CSB-EP429117AYS
CSB-BP429117AYS
CSB-MP429117AYS
CSB-EP429117AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myrmecia banksi Pilosulin-5 (Myrb5) CSB-YP429118MOS
CSB-EP429118MOS
CSB-BP429118MOS
CSB-MP429118MOS
CSB-EP429118MOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterocytozoon bieneusi Probable lysine--tRNA ligase, cytoplasmic (EBI_25548), partial CSB-YP429119EMA
CSB-EP429119EMA
CSB-BP429119EMA
CSB-MP429119EMA
CSB-EP429119EMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>