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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella baltica Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP429300STN
CSB-EP429300STN
CSB-BP429300STN
CSB-MP429300STN
CSB-EP429300STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Imidazolonepropionase (hutI) CSB-YP429301STN
CSB-EP429301STN
CSB-BP429301STN
CSB-MP429301STN
CSB-EP429301STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Homoserine O-succinyltransferase (metA) CSB-YP429302STN
CSB-EP429302STN
CSB-BP429302STN
CSB-MP429302STN
CSB-EP429302STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP429303STN
CSB-EP429303STN
CSB-BP429303STN
CSB-MP429303STN
CSB-EP429303STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Elongation factor Tu 2 (tuf2) CSB-YP429304STN
CSB-EP429304STN
CSB-BP429304STN
CSB-MP429304STN
CSB-EP429304STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S3 (rpsC) CSB-YP429305STN
CSB-EP429305STN
CSB-BP429305STN
CSB-MP429305STN
CSB-EP429305STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L15 (rplO) CSB-YP429306STN
CSB-EP429306STN
CSB-BP429306STN
CSB-MP429306STN
CSB-EP429306STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP429307STN
CSB-EP429307STN
CSB-BP429307STN
CSB-MP429307STN
CSB-EP429307STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Bifunctional protein GlmU (glmU) CSB-YP429308STN
CSB-EP429308STN
CSB-BP429308STN
CSB-MP429308STN
CSB-EP429308STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP synthase subunit c (atpE), partial CSB-YP429309STN1
CSB-EP429309STN1
CSB-BP429309STN1
CSB-MP429309STN1
CSB-EP429309STN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribonuclease P protein component (rnpA) CSB-YP429310STN
CSB-EP429310STN
CSB-BP429310STN
CSB-MP429310STN
CSB-EP429310STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica D-tyrosyl-tRNA (Tyr) deacylase CSB-YP429311STN
CSB-EP429311STN
CSB-BP429311STN
CSB-MP429311STN
CSB-EP429311STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L32 (rpmF) CSB-YP429312STN
CSB-EP429312STN
CSB-BP429312STN
CSB-MP429312STN
CSB-EP429312STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0301 protein Sbal195_3177 (Sbal195_3177) CSB-YP429313STN
CSB-EP429313STN
CSB-BP429313STN
CSB-MP429313STN
CSB-EP429313STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 2-isopropylmalate synthase (leuA), partial CSB-YP429314STN
CSB-EP429314STN
CSB-BP429314STN
CSB-MP429314STN
CSB-EP429314STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP429315STN
CSB-EP429315STN
CSB-BP429315STN
CSB-MP429315STN
CSB-EP429315STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Biotin synthase (bioB) CSB-YP429316STN
CSB-EP429316STN
CSB-BP429316STN
CSB-MP429316STN
CSB-EP429316STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein RecA (recA) CSB-YP429317STN
CSB-EP429317STN
CSB-BP429317STN
CSB-MP429317STN
CSB-EP429317STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP429318STN
CSB-EP429318STN
CSB-BP429318STN
CSB-MP429318STN
CSB-EP429318STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP429319STN
CSB-EP429319STN
CSB-BP429319STN
CSB-MP429319STN
CSB-EP429319STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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