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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Petrotoga mobilis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP429080PYI
CSB-EP429080PYI
CSB-BP429080PYI
CSB-MP429080PYI
CSB-EP429080PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Adenine deaminase (ade), partial CSB-YP429081PYI
CSB-EP429081PYI
CSB-BP429081PYI
CSB-MP429081PYI
CSB-EP429081PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Gamma-glutamyl phosphate reductase (proA) CSB-YP429082PYI
CSB-EP429082PYI
CSB-BP429082PYI
CSB-MP429082PYI
CSB-EP429082PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis D-ribose pyranase (rbsD) CSB-YP429083PYI
CSB-EP429083PYI
CSB-BP429083PYI
CSB-MP429083PYI
CSB-EP429083PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis GTPase obg (obg) CSB-YP429084PYI
CSB-EP429084PYI
CSB-BP429084PYI
CSB-MP429084PYI
CSB-EP429084PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Putative arginyl-tRNA--protein transferase (ate) CSB-YP429085DHV
CSB-EP429085DHV
CSB-BP429085DHV
CSB-MP429085DHV
CSB-EP429085DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Ribonuclease HII (rnhB) CSB-YP429086DHV
CSB-EP429086DHV
CSB-BP429086DHV
CSB-MP429086DHV
CSB-EP429086DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans UPF0317 protein Daci_4977 (Daci_4977) CSB-YP429087DHV
CSB-EP429087DHV
CSB-BP429087DHV
CSB-MP429087DHV
CSB-EP429087DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP429088DHV
CSB-EP429088DHV
CSB-BP429088DHV
CSB-MP429088DHV
CSB-EP429088DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Elongation factor 4 (lepA), partial CSB-YP429089DHV
CSB-EP429089DHV
CSB-BP429089DHV
CSB-MP429089DHV
CSB-EP429089DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Ribosome maturation factor RimM (rimM) CSB-YP429090DHV
CSB-EP429090DHV
CSB-BP429090DHV
CSB-MP429090DHV
CSB-EP429090DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L33 (rpmG) CSB-YP429091DHV
CSB-EP429091DHV
CSB-BP429091DHV
CSB-MP429091DHV
CSB-EP429091DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Peptidyl-tRNA hydrolase (pth) CSB-YP429092DHV
CSB-EP429092DHV
CSB-BP429092DHV
CSB-MP429092DHV
CSB-EP429092DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L27 (rpmA) CSB-YP429093DHV
CSB-EP429093DHV
CSB-BP429093DHV
CSB-MP429093DHV
CSB-EP429093DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L3 (rplC) CSB-YP429094DHV
CSB-EP429094DHV
CSB-BP429094DHV
CSB-MP429094DHV
CSB-EP429094DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 30S ribosomal protein S17 (rpsQ) CSB-YP429095DHV
CSB-EP429095DHV
CSB-BP429095DHV
CSB-MP429095DHV
CSB-EP429095DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP429096DHV
CSB-EP429096DHV
CSB-BP429096DHV
CSB-MP429096DHV
CSB-EP429096DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L1 (rplA) CSB-YP429097DHV
CSB-EP429097DHV
CSB-BP429097DHV
CSB-MP429097DHV
CSB-EP429097DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L24 (rplX) CSB-YP429098DHV
CSB-EP429098DHV
CSB-BP429098DHV
CSB-MP429098DHV
CSB-EP429098DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 30S ribosomal protein S8 (rpsH) CSB-YP429099DHV
CSB-EP429099DHV
CSB-BP429099DHV
CSB-MP429099DHV
CSB-EP429099DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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