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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Petrotoga mobilis 50S ribosomal protein L2 (rplB) CSB-YP430882PYI
CSB-EP430882PYI
CSB-BP430882PYI
CSB-MP430882PYI
CSB-EP430882PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 50S ribosomal protein L13 (rplM) CSB-YP430883PYI
CSB-EP430883PYI
CSB-BP430883PYI
CSB-MP430883PYI
CSB-EP430883PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP430884PYI
CSB-EP430884PYI
CSB-BP430884PYI
CSB-MP430884PYI
CSB-EP430884PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Acylphosphatase (acyP) CSB-YP430885PYI
CSB-EP430885PYI
CSB-BP430885PYI
CSB-MP430885PYI
CSB-EP430885PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Probable GTP-binding protein EngB (engB) CSB-YP430886PYI
CSB-EP430886PYI
CSB-BP430886PYI
CSB-MP430886PYI
CSB-EP430886PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis tRNA modification GTPase MnmE (mnmE) CSB-YP430887PYI
CSB-EP430887PYI
CSB-BP430887PYI
CSB-MP430887PYI
CSB-EP430887PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP430888PYI
CSB-EP430888PYI
CSB-BP430888PYI
CSB-MP430888PYI
CSB-EP430888PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP430889PYI
CSB-EP430889PYI
CSB-BP430889PYI
CSB-MP430889PYI
CSB-EP430889PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 50S ribosomal protein L31 (rpmE) CSB-YP430890PYI
CSB-EP430890PYI
CSB-BP430890PYI
CSB-MP430890PYI
CSB-EP430890PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Ribonuclease P protein component (rnpA) CSB-YP430891PYI
CSB-EP430891PYI
CSB-BP430891PYI
CSB-MP430891PYI
CSB-EP430891PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis tRNA (Ile)-lysidine synthase CSB-YP430892PYI
CSB-EP430892PYI
CSB-BP430892PYI
CSB-MP430892PYI
CSB-EP430892PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis DNA mismatch repair protein MutS (mutS), partial CSB-YP430893PYI
CSB-EP430893PYI
CSB-BP430893PYI
CSB-MP430893PYI
CSB-EP430893PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Glutamate--tRNA ligase (gltX) CSB-YP430894DHV
CSB-EP430894DHV
CSB-BP430894DHV
CSB-MP430894DHV
CSB-EP430894DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 30S ribosomal protein S2 (rpsB) CSB-YP430895DHV
CSB-EP430895DHV
CSB-BP430895DHV
CSB-MP430895DHV
CSB-EP430895DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans GTPase Der (der) CSB-YP430896DHV
CSB-EP430896DHV
CSB-BP430896DHV
CSB-MP430896DHV
CSB-EP430896DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 30S ribosomal protein S15 (rpsO) CSB-YP430897DHV
CSB-EP430897DHV
CSB-BP430897DHV
CSB-MP430897DHV
CSB-EP430897DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Chaperone protein DnaJ (dnaJ) CSB-YP430898DHV
CSB-EP430898DHV
CSB-BP430898DHV
CSB-MP430898DHV
CSB-EP430898DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans DNA repair protein recO (recO) CSB-YP430899DHV
CSB-EP430899DHV
CSB-BP430899DHV
CSB-MP430899DHV
CSB-EP430899DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP430900DHV
CSB-EP430900DHV
CSB-BP430900DHV
CSB-MP430900DHV
CSB-EP430900DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans UPF0042 nucleotide-binding protein Daci_5422 (Daci_5422) CSB-YP430901DHV
CSB-EP430901DHV
CSB-BP430901DHV
CSB-MP430901DHV
CSB-EP430901DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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