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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii SsrA-binding protein (smpB) CSB-YP429260DXO
CSB-EP429260DXO
CSB-BP429260DXO
CSB-MP429260DXO
CSB-EP429260DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP429261DXO
CSB-EP429261DXO
CSB-BP429261DXO
CSB-MP429261DXO
CSB-EP429261DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Chaperone protein htpG (htpG), partial CSB-YP429262DXO
CSB-EP429262DXO
CSB-BP429262DXO
CSB-MP429262DXO
CSB-EP429262DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP429263DXO
CSB-EP429263DXO
CSB-BP429263DXO
CSB-MP429263DXO
CSB-EP429263DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Bis (5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) CSB-YP429264DXO
CSB-BP429264DXO
CSB-MP429264DXO
CSB-EP429264DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Protein smg homolog (smg) CSB-YP429265DXO
CSB-EP429265DXO
CSB-BP429265DXO
CSB-MP429265DXO
CSB-EP429265DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 50S ribosomal protein L35 (rpmI) CSB-YP429266CWZ
CSB-EP429266CWZ
CSB-BP429266CWZ
CSB-MP429266CWZ
CSB-EP429266CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP429267CWZ
CSB-EP429267CWZ
CSB-BP429267CWZ
CSB-MP429267CWZ
CSB-EP429267CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Transcription elongation factor GreA (greA) CSB-YP429268CWZ
CSB-EP429268CWZ
CSB-BP429268CWZ
CSB-MP429268CWZ
CSB-EP429268CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 30S ribosomal protein S11 (rpsK) CSB-YP429269CWZ
CSB-EP429269CWZ
CSB-BP429269CWZ
CSB-MP429269CWZ
CSB-EP429269CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 50S ribosomal protein L22 (rplV) CSB-YP429270CWZ
CSB-EP429270CWZ
CSB-BP429270CWZ
CSB-MP429270CWZ
CSB-EP429270CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 50S ribosomal protein L3 (rplC) CSB-YP429271CWZ
CSB-EP429271CWZ
CSB-BP429271CWZ
CSB-MP429271CWZ
CSB-EP429271CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Urease accessory protein UreG (ureG) CSB-YP429272CWZ
CSB-EP429272CWZ
CSB-BP429272CWZ
CSB-MP429272CWZ
CSB-EP429272CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Urease subunit gamma (ureA) CSB-YP429273CWZ
CSB-EP429273CWZ
CSB-BP429273CWZ
CSB-MP429273CWZ
CSB-EP429273CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans ATP synthase gamma chain (atpG) CSB-YP429274CWZ
CSB-EP429274CWZ
CSB-BP429274CWZ
CSB-MP429274CWZ
CSB-EP429274CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Probable tRNA sulfurtransferase (thiI) CSB-YP429275CWZ
CSB-EP429275CWZ
CSB-BP429275CWZ
CSB-MP429275CWZ
CSB-EP429275CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Elongation factor 4 (lepA), partial CSB-YP429276CWZ
CSB-EP429276CWZ
CSB-BP429276CWZ
CSB-MP429276CWZ
CSB-EP429276CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP429277CWZ
CSB-EP429277CWZ
CSB-BP429277CWZ
CSB-MP429277CWZ
CSB-EP429277CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans GTPase obg (obg) CSB-YP429278CWZ
CSB-EP429278CWZ
CSB-BP429278CWZ
CSB-MP429278CWZ
CSB-EP429278CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Gamma-glutamyl phosphate reductase (proA) CSB-YP429279CWZ
CSB-EP429279CWZ
CSB-BP429279CWZ
CSB-MP429279CWZ
CSB-EP429279CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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