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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Agrobacterium tumefaciens DNA ligase (ligA), partial CSB-YP430922AYS
CSB-EP430922AYS
CSB-BP430922AYS
CSB-MP430922AYS
CSB-EP430922AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative tail protein (orf50) CSB-YP430923SUN
CSB-EP430923SUN
CSB-BP430923SUN
CSB-MP430923SUN
CSB-EP430923SUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L35 (rpmI) CSB-YP430924SUD
CSB-EP430924SUD
CSB-BP430924SUD
CSB-MP430924SUD
CSB-EP430924SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L2 (rplB) CSB-YP430925SUD
CSB-EP430925SUD
CSB-BP430925SUD
CSB-MP430925SUD
CSB-EP430925SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Thiazole synthase (thiG) CSB-YP430926SUD
CSB-EP430926SUD
CSB-BP430926SUD
CSB-MP430926SUD
CSB-EP430926SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Leucine--tRNA ligase (leuS), partial CSB-YP430927SUD
CSB-EP430927SUD
CSB-BP430927SUD
CSB-MP430927SUD
CSB-EP430927SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 30S ribosomal protein S16 (rpsP) CSB-YP430928SUD
CSB-EP430928SUD
CSB-BP430928SUD
CSB-MP430928SUD
CSB-EP430928SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Histidine--tRNA ligase (hisS) CSB-YP430929SUD
CSB-EP430929SUD
CSB-BP430929SUD
CSB-MP430929SUD
CSB-EP430929SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP430930SUD
CSB-EP430930SUD
CSB-BP430930SUD
CSB-MP430930SUD
CSB-EP430930SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Adenylate kinase (adk) CSB-YP430931SUD
CSB-EP430931SUD
CSB-BP430931SUD
CSB-MP430931SUD
CSB-EP430931SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Dihydrodipicolinate synthase (dapA) CSB-YP430932SUD
CSB-EP430932SUD
CSB-BP430932SUD
CSB-MP430932SUD
CSB-EP430932SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L33 3 (rpmG3) CSB-YP430933SUD
CSB-EP430933SUD
CSB-BP430933SUD
CSB-MP430933SUD
CSB-EP430933SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Pantothenate kinase (coaA) CSB-YP430934SUD
CSB-EP430934SUD
CSB-BP430934SUD
CSB-MP430934SUD
CSB-EP430934SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L33 2 (rpmG2) CSB-YP430935SUD
CSB-EP430935SUD
CSB-BP430935SUD
CSB-MP430935SUD
CSB-EP430935SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP430936SUD
CSB-EP430936SUD
CSB-BP430936SUD
CSB-MP430936SUD
CSB-EP430936SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP430937SUD
CSB-EP430937SUD
CSB-BP430937SUD
CSB-MP430937SUD
CSB-EP430937SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP430938SUD
CSB-EP430938SUD
CSB-BP430938SUD
CSB-MP430938SUD
CSB-EP430938SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Heat-inducible transcription repressor HrcA (hrcA) CSB-YP430939SUD
CSB-EP430939SUD
CSB-BP430939SUD
CSB-MP430939SUD
CSB-EP430939SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP430940SUD
CSB-EP430940SUD
CSB-BP430940SUD
CSB-MP430940SUD
CSB-EP430940SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Urease subunit alpha (ureC), partial CSB-YP430941SUD
CSB-EP430941SUD
CSB-BP430941SUD
CSB-MP430941SUD
CSB-EP430941SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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