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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis bv. Antiqua Homoserine kinase (thrB) CSB-YP429660YAM
CSB-EP429660YAM
CSB-BP429660YAM
CSB-MP429660YAM
CSB-EP429660YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Autoinducer 2 import system permease protein lsrC (lsrC), partial CSB-YP429661YAM
CSB-EP429661YAM
CSB-BP429661YAM
CSB-MP429661YAM
CSB-EP429661YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP429662YAM
CSB-EP429662YAM
CSB-BP429662YAM
CSB-MP429662YAM
CSB-EP429662YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L20 (rplT) CSB-YP429663YAM
CSB-EP429663YAM
CSB-BP429663YAM
CSB-MP429663YAM
CSB-EP429663YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0227 protein YpAngola_A2819 (YpAngola_A2819) CSB-YP429664YAM
CSB-EP429664YAM
CSB-BP429664YAM
CSB-MP429664YAM
CSB-EP429664YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Protein translocase subunit SecA (secA), partial CSB-YP429665YAM
CSB-EP429665YAM
CSB-BP429665YAM
CSB-MP429665YAM
CSB-EP429665YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua S-ribosylhomocysteine lyase (luxS) CSB-YP429666YAM
CSB-EP429666YAM
CSB-BP429666YAM
CSB-MP429666YAM
CSB-EP429666YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP429667YAM
CSB-EP429667YAM
CSB-BP429667YAM
CSB-MP429667YAM
CSB-EP429667YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua H (+)/Cl (-) exchange transporter ClcA, partial CSB-YP429668YAM
CSB-EP429668YAM
CSB-BP429668YAM
CSB-MP429668YAM
CSB-EP429668YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0102 protein YpAngola_A1121 (YpAngola_A1121) CSB-YP429669YAM
CSB-EP429669YAM
CSB-BP429669YAM
CSB-MP429669YAM
CSB-EP429669YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP429670YAM
CSB-EP429670YAM
CSB-BP429670YAM
CSB-MP429670YAM
CSB-EP429670YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP429671YAM
CSB-EP429671YAM
CSB-BP429671YAM
CSB-MP429671YAM
CSB-EP429671YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua DNA-binding protein fis (fis) CSB-YP429672YAM
CSB-EP429672YAM
CSB-BP429672YAM
CSB-MP429672YAM
CSB-EP429672YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP429673YAM
CSB-EP429673YAM
CSB-BP429673YAM
CSB-MP429673YAM
CSB-EP429673YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP429674YAM
CSB-EP429674YAM
CSB-BP429674YAM
CSB-MP429674YAM
CSB-EP429674YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Histidinol-phosphate aminotransferase (hisC) CSB-YP429675YAM
CSB-EP429675YAM
CSB-BP429675YAM
CSB-MP429675YAM
CSB-EP429675YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Methylglyoxal synthase (mgsA) CSB-YP429676YAM
CSB-EP429676YAM
CSB-BP429676YAM
CSB-MP429676YAM
CSB-EP429676YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Recombination-associated protein rdgC (rdgC) CSB-YP429677YAM
CSB-EP429677YAM
CSB-BP429677YAM
CSB-MP429677YAM
CSB-EP429677YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Gamma-glutamyl phosphate reductase (proA) CSB-YP429678YAM
CSB-EP429678YAM
CSB-BP429678YAM
CSB-MP429678YAM
CSB-EP429678YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Na (+)-translocating NADH-quinone reductase subunit A CSB-YP429679YAM
CSB-EP429679YAM
CSB-BP429679YAM
CSB-MP429679YAM
CSB-EP429679YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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