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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sorangium cellulosum 50S ribosomal protein L23 (rplW) CSB-YP430041SUD
CSB-EP430041SUD
CSB-BP430041SUD
CSB-MP430041SUD
CSB-EP430041SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum ATP-dependent zinc metalloprotease FtsH 4 (ftsH4), partial CSB-YP430042SUD
CSB-EP430042SUD
CSB-BP430042SUD
CSB-MP430042SUD
CSB-EP430042SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Ribosome maturation factor RimM (rimM) CSB-YP430043SUD
CSB-EP430043SUD
CSB-BP430043SUD
CSB-MP430043SUD
CSB-EP430043SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP430044SUD
CSB-EP430044SUD
CSB-BP430044SUD
CSB-MP430044SUD
CSB-EP430044SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 30S ribosomal protein S15 (rpsO) CSB-YP430045SUD
CSB-EP430045SUD
CSB-BP430045SUD
CSB-MP430045SUD
CSB-EP430045SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Acireductone dioxygenase (mtnD) CSB-YP430046SUD
CSB-EP430046SUD
CSB-BP430046SUD
CSB-MP430046SUD
CSB-EP430046SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum ATP-dependent zinc metalloprotease FtsH 2 (ftsH2), partial CSB-YP430047SUD
CSB-EP430047SUD
CSB-BP430047SUD
CSB-MP430047SUD
CSB-EP430047SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L17 (rplQ) CSB-YP430048SUD
CSB-EP430048SUD
CSB-BP430048SUD
CSB-MP430048SUD
CSB-EP430048SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 30S ribosomal protein S8 (rpsH) CSB-YP430049SUD
CSB-EP430049SUD
CSB-BP430049SUD
CSB-MP430049SUD
CSB-EP430049SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L22 (rplV) CSB-YP430050SUD
CSB-EP430050SUD
CSB-BP430050SUD
CSB-MP430050SUD
CSB-EP430050SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP430051SUD
CSB-EP430051SUD
CSB-BP430051SUD
CSB-MP430051SUD
CSB-EP430051SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Alanine--tRNA ligase (alaS), partial CSB-YP430052SUD
CSB-EP430052SUD
CSB-BP430052SUD
CSB-MP430052SUD
CSB-EP430052SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Fructose-1,6-bisphosphatase class 3 (fbp), partial CSB-YP430053SUD
CSB-EP430053SUD
CSB-BP430053SUD
CSB-MP430053SUD
CSB-EP430053SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L19 (rplS) CSB-YP430054SUD
CSB-EP430054SUD
CSB-BP430054SUD
CSB-MP430054SUD
CSB-EP430054SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Phosphoglucosamine mutase (glmM) CSB-YP430055SUD
CSB-EP430055SUD
CSB-BP430055SUD
CSB-MP430055SUD
CSB-EP430055SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 60 kDa chaperonin 1 (groL1), partial CSB-YP430056SUD
CSB-EP430056SUD
CSB-BP430056SUD
CSB-MP430056SUD
CSB-EP430056SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Undecaprenyl-diphosphatase (uppP), partial CSB-YP430057SUD1
CSB-EP430057SUD1
CSB-BP430057SUD1
CSB-MP430057SUD1
CSB-EP430057SUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum UPF0102 protein sce2912 (sce2912) CSB-YP430058SUD
CSB-EP430058SUD
CSB-BP430058SUD
CSB-MP430058SUD
CSB-EP430058SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 30S ribosomal protein S2 (rpsB) CSB-YP430059SUD
CSB-EP430059SUD
CSB-BP430059SUD
CSB-MP430059SUD
CSB-EP430059SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Na (+)/H (+) antiporter nhaA 1, partial CSB-YP430060SUD
CSB-EP430060SUD
CSB-BP430060SUD
CSB-MP430060SUD
CSB-EP430060SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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