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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella suis ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP431622BWU
CSB-EP431622BWU
CSB-BP431622BWU
CSB-MP431622BWU
CSB-EP431622BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis DNA mismatch repair protein mutL (mutL), partial CSB-YP431623BWU
CSB-EP431623BWU
CSB-BP431623BWU
CSB-MP431623BWU
CSB-EP431623BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP431624BWU
CSB-EP431624BWU
CSB-BP431624BWU
CSB-MP431624BWU
CSB-EP431624BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Putative nickel-responsive regulator (BSUIS_B0797) CSB-YP431625BWU
CSB-EP431625BWU
CSB-BP431625BWU
CSB-MP431625BWU
CSB-EP431625BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio anubis 39S ribosomal protein L9, mitochondrial (MRPL9), partial CSB-YP431626ERI
CSB-EP431626ERI
CSB-BP431626ERI
CSB-MP431626ERI
CSB-EP431626ERI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buthus eupeus Potassium channel toxin MeuTXK-beta-1 CSB-YP431627BPW
CSB-EP431627BPW
CSB-BP431627BPW
CSB-MP431627BPW
CSB-EP431627BPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carica papaya Protein psbN (psbN), partial CSB-YP431628DQH
CSB-EP431628DQH
CSB-BP431628DQH
CSB-MP431628DQH
CSB-EP431628DQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus DNA polymerase IV (dbh) CSB-YP431629NFB
CSB-EP431629NFB
CSB-BP431629NFB
CSB-MP431629NFB
CSB-EP431629NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus S-methyl-5'-thioadenosine phosphorylase (mtnP) CSB-YP431630NFB
CSB-EP431630NFB
CSB-BP431630NFB
CSB-MP431630NFB
CSB-EP431630NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Sulfate adenylyltransferase (sat) CSB-YP431631NFB
CSB-EP431631NFB
CSB-BP431631NFB
CSB-MP431631NFB
CSB-EP431631NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Probable exosome complex RNA-binding protein 1 (Nmar_0431) CSB-YP431632NFB
CSB-EP431632NFB
CSB-BP431632NFB
CSB-MP431632NFB
CSB-EP431632NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Ribonuclease P protein component 1 (rnp1) CSB-YP431633NFB
CSB-EP431633NFB
CSB-BP431633NFB
CSB-MP431633NFB
CSB-EP431633NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP431634NFB
CSB-EP431634NFB
CSB-BP431634NFB
CSB-MP431634NFB
CSB-EP431634NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Cytidylate kinase (cmk) CSB-YP431635MNQ
CSB-EP431635MNQ
CSB-BP431635MNQ
CSB-MP431635MNQ
CSB-EP431635MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 1A (eIF1A) CSB-YP431636MNQ
CSB-EP431636MNQ
CSB-BP431636MNQ
CSB-MP431636MNQ
CSB-EP431636MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease P protein component 3 (rnp3) CSB-YP431637MNQ
CSB-EP431637MNQ
CSB-BP431637MNQ
CSB-MP431637MNQ
CSB-EP431637MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP431638MNQ
CSB-EP431638MNQ
CSB-BP431638MNQ
CSB-MP431638MNQ
CSB-EP431638MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP431639MNQ
CSB-EP431639MNQ
CSB-BP431639MNQ
CSB-MP431639MNQ
CSB-EP431639MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative HTH-type transcriptional regulatory protein MmarC6_0210 (MmarC6_0210) CSB-YP431640MNQ
CSB-EP431640MNQ
CSB-BP431640MNQ
CSB-MP431640MNQ
CSB-EP431640MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP431641MNQ
CSB-EP431641MNQ
CSB-BP431641MNQ
CSB-MP431641MNQ
CSB-EP431641MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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