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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lemna minor DNA-directed RNA polymerase subunit beta' (rpoC1), partial CSB-YP430262LLL
CSB-EP430262LLL
CSB-BP430262LLL
CSB-MP430262LLL
CSB-EP430262LLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemna minor NAD (P)H-quinone oxidoreductase subunit 3, chloroplastic, partial CSB-YP430263LLL1
CSB-EP430263LLL1
CSB-BP430263LLL1
CSB-MP430263LLL1
CSB-EP430263LLL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemna minor Photosystem I reaction center subunit IX (psaJ), partial CSB-YP430264LLL
CSB-EP430264LLL
CSB-BP430264LLL
CSB-MP430264LLL
CSB-EP430264LLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemna minor 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP430265LLL
CSB-EP430265LLL
CSB-BP430265LLL
CSB-MP430265LLL
CSB-EP430265LLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemna minor NAD (P)H-quinone oxidoreductase subunit 5, chloroplastic, partial CSB-YP430266LLL
CSB-EP430266LLL
CSB-BP430266LLL
CSB-MP430266LLL
CSB-EP430266LLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Wilms tumor protein 1-interacting protein homolog (wtip), partial CSB-YP430267XBE
CSB-EP430267XBE
CSB-BP430267XBE
CSB-MP430267XBE
CSB-EP430267XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acorus americanus 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP430268AUE
CSB-EP430268AUE
CSB-BP430268AUE
CSB-MP430268AUE
CSB-EP430268AUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acorus americanus Putative membrane protein ycf1 (ycf1), partial CSB-YP430269AUE
CSB-EP430269AUE
CSB-BP430269AUE
CSB-MP430269AUE
CSB-EP430269AUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP430270NEW1
CSB-EP430270NEW1
CSB-BP430270NEW1
CSB-MP430270NEW1
CSB-EP430270NEW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C UPF0758 protein NMCC_1157 (NMCC_1157) CSB-YP430271NEW
CSB-EP430271NEW
CSB-BP430271NEW
CSB-MP430271NEW
CSB-EP430271NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP430272NEW
CSB-EP430272NEW
CSB-BP430272NEW
CSB-MP430272NEW
CSB-EP430272NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C 30S ribosomal protein S6 (rpsF) CSB-YP430273NEW
CSB-EP430273NEW
CSB-BP430273NEW
CSB-MP430273NEW
CSB-EP430273NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C Putative Holliday junction resolvase (NMCC_1250) CSB-YP430274NEW
CSB-EP430274NEW
CSB-BP430274NEW
CSB-MP430274NEW
CSB-EP430274NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP430275NEW
CSB-EP430275NEW
CSB-BP430275NEW
CSB-MP430275NEW
CSB-EP430275NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C Thiazole synthase (thiG) CSB-YP430276NEW
CSB-EP430276NEW
CSB-BP430276NEW
CSB-MP430276NEW
CSB-EP430276NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C 5'-nucleotidase surE (surE) CSB-YP430277NEW
CSB-EP430277NEW
CSB-BP430277NEW
CSB-MP430277NEW
CSB-EP430277NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C Alanine--tRNA ligase (alaS), partial CSB-YP430278NEW
CSB-EP430278NEW
CSB-BP430278NEW
CSB-MP430278NEW
CSB-EP430278NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP430279NEW
CSB-EP430279NEW
CSB-BP430279NEW
CSB-MP430279NEW
CSB-EP430279NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP430280NEW
CSB-EP430280NEW
CSB-BP430280NEW
CSB-MP430280NEW
CSB-EP430280NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C 3-dehydroquinate synthase (aroB) CSB-YP430281NEW
CSB-EP430281NEW
CSB-BP430281NEW
CSB-MP430281NEW
CSB-EP430281NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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