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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella paratyphi B 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP432203STF
CSB-EP432203STF
CSB-BP432203STF
CSB-MP432203STF
CSB-EP432203STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Probable crotonobetaine/carnitine-CoA ligase (caiC), partial CSB-YP432204STF
CSB-EP432204STF
CSB-BP432204STF
CSB-MP432204STF
CSB-EP432204STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Protein ApaG (apaG) CSB-YP432205STF
CSB-EP432205STF
CSB-BP432205STF
CSB-MP432205STF
CSB-EP432205STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Probable sugar efflux transporter (sotB), partial CSB-YP432206STF
CSB-EP432206STF
CSB-BP432206STF
CSB-MP432206STF
CSB-EP432206STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B HTH-type transcriptional activator rhaR (rhaR) CSB-YP432207STF
CSB-EP432207STF
CSB-BP432207STF
CSB-MP432207STF
CSB-EP432207STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Transcriptional regulator lsrR (lsrR) CSB-YP432208STF
CSB-EP432208STF
CSB-BP432208STF
CSB-MP432208STF
CSB-EP432208STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Glycerol kinase (glpK), partial CSB-YP432209STF
CSB-EP432209STF
CSB-BP432209STF
CSB-MP432209STF
CSB-EP432209STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP432210STF
CSB-EP432210STF
CSB-BP432210STF
CSB-MP432210STF
CSB-EP432210STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Electron transport complex protein RnfA (rnfA), partial CSB-YP432211STF1
CSB-EP432211STF1
CSB-BP432211STF1
CSB-MP432211STF1
CSB-EP432211STF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP432212STF
CSB-EP432212STF
CSB-BP432212STF
CSB-MP432212STF
CSB-EP432212STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Alanine--tRNA ligase (alaS), partial CSB-YP432213STF
CSB-EP432213STF
CSB-BP432213STF
CSB-MP432213STF
CSB-EP432213STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Anaerobic nitric oxide reductase transcription regulator norR (norR), partial CSB-YP432214STF
CSB-EP432214STF
CSB-BP432214STF
CSB-MP432214STF
CSB-EP432214STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP432215STF
CSB-EP432215STF
CSB-BP432215STF
CSB-MP432215STF
CSB-EP432215STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 50S ribosomal protein L7/L12 (rplL) CSB-YP432216STF
CSB-EP432216STF
CSB-BP432216STF
CSB-MP432216STF
CSB-EP432216STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Thiazole synthase (thiG) CSB-YP432217STF
CSB-EP432217STF
CSB-BP432217STF
CSB-MP432217STF
CSB-EP432217STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B H (+)/Cl (-) exchange transporter ClcA, partial CSB-YP432218STF
CSB-EP432218STF
CSB-BP432218STF
CSB-MP432218STF
CSB-EP432218STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP432219STF
CSB-EP432219STF
CSB-BP432219STF
CSB-MP432219STF
CSB-EP432219STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Quinate/shikimate dehydrogenase (ydiB) CSB-YP432220STF
CSB-EP432220STF
CSB-BP432220STF
CSB-MP432220STF
CSB-EP432220STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Cation/acetate symporter ActP (actP), partial CSB-YP432221STF
CSB-EP432221STF
CSB-BP432221STF
CSB-MP432221STF
CSB-EP432221STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Co-chaperone protein hscB (hscB) CSB-YP432222STF
CSB-EP432222STF
CSB-BP432222STF
CSB-MP432222STF
CSB-EP432222STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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