Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella canis Methylglyoxal synthase (mgsA) CSB-YP430342BPH
CSB-EP430342BPH
CSB-BP430342BPH
CSB-MP430342BPH
CSB-EP430342BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP430343BPH
CSB-EP430343BPH
CSB-BP430343BPH
CSB-MP430343BPH
CSB-EP430343BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Cysteine desulfuration protein sufE (sufE) CSB-YP430344STE
CSB-EP430344STE
CSB-BP430344STE
CSB-MP430344STE
CSB-EP430344STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Cell division protein FtsB (ftsB), partial CSB-YP430345STE
CSB-EP430345STE
CSB-BP430345STE
CSB-MP430345STE
CSB-EP430345STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae DNA mismatch repair protein MutS (mutS), partial CSB-YP430346STE
CSB-EP430346STE
CSB-BP430346STE
CSB-MP430346STE
CSB-EP430346STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 50S ribosomal protein L35 (rpmI) CSB-YP430347STE
CSB-EP430347STE
CSB-BP430347STE
CSB-MP430347STE
CSB-EP430347STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP430348STE
CSB-EP430348STE
CSB-BP430348STE
CSB-MP430348STE
CSB-EP430348STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0756 membrane protein YeaL (yeaL), partial CSB-YP430349STE1
CSB-EP430349STE1
CSB-BP430349STE1
CSB-MP430349STE1
CSB-EP430349STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae DNA mismatch repair protein mutL (mutL), partial CSB-YP430350STE
CSB-EP430350STE
CSB-BP430350STE
CSB-MP430350STE
CSB-EP430350STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 60 kDa chaperonin (groL), partial CSB-YP430351STE
CSB-EP430351STE
CSB-BP430351STE
CSB-MP430351STE
CSB-EP430351STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Protein nrdI (nrdI) CSB-YP430352STE
CSB-EP430352STE
CSB-BP430352STE
CSB-MP430352STE
CSB-EP430352STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Cytochrome c-552 (nrfA) CSB-YP430353STE
CSB-EP430353STE
CSB-BP430353STE
CSB-MP430353STE
CSB-EP430353STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae SsrA-binding protein (smpB) CSB-YP430354STE
CSB-EP430354STE
CSB-BP430354STE
CSB-MP430354STE
CSB-EP430354STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP430355STE
CSB-EP430355STE
CSB-BP430355STE
CSB-MP430355STE
CSB-EP430355STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0502 protein YceH (yceH) CSB-YP430356STE
CSB-EP430356STE
CSB-BP430356STE
CSB-MP430356STE
CSB-EP430356STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glyoxylate/hydroxypyruvate reductase A (ghrA) CSB-YP430357STE
CSB-EP430357STE
CSB-BP430357STE
CSB-MP430357STE
CSB-EP430357STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Chorismate--pyruvate lyase (ubiC) CSB-YP430358STE
CSB-EP430358STE
CSB-BP430358STE
CSB-MP430358STE
CSB-EP430358STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Phosphomethylpyrimidine synthase (thiC), partial CSB-YP430359STE
CSB-EP430359STE
CSB-BP430359STE
CSB-MP430359STE
CSB-EP430359STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae tRNA (uracil (54)-C (5))-methyltransferase CSB-YP430360STE
CSB-EP430360STE
CSB-BP430360STE
CSB-MP430360STE
CSB-EP430360STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Iron-binding protein IscA (iscA) CSB-YP430361STE
CSB-EP430361STE
CSB-BP430361STE
CSB-MP430361STE
CSB-EP430361STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>