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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis 50S ribosomal protein L1P (rpl1p) CSB-YP431642MNQ
CSB-EP431642MNQ
CSB-BP431642MNQ
CSB-MP431642MNQ
CSB-EP431642MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP431643MNQ
CSB-EP431643MNQ
CSB-BP431643MNQ
CSB-MP431643MNQ
CSB-EP431643MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L40e (rpl40e) CSB-YP431644MNQ
CSB-EP431644MNQ
CSB-BP431644MNQ
CSB-MP431644MNQ
CSB-EP431644MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamyl-tRNA reductase (hemA) CSB-YP431645MNQ
CSB-EP431645MNQ
CSB-BP431645MNQ
CSB-MP431645MNQ
CSB-EP431645MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP431646MNQ
CSB-EP431646MNQ
CSB-BP431646MNQ
CSB-MP431646MNQ
CSB-EP431646MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP431647MNQ
CSB-EP431647MNQ
CSB-BP431647MNQ
CSB-MP431647MNQ
CSB-EP431647MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Aspartate carbamoyltransferase (pyrB) CSB-YP431648MNQ
CSB-EP431648MNQ
CSB-BP431648MNQ
CSB-MP431648MNQ
CSB-EP431648MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L2P (rpl2p) CSB-YP431649MNQ
CSB-EP431649MNQ
CSB-BP431649MNQ
CSB-MP431649MNQ
CSB-EP431649MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis GMP synthase [glutamine-hydrolyzing] subunit A (guaAA) CSB-YP431650MNQ
CSB-EP431650MNQ
CSB-BP431650MNQ
CSB-MP431650MNQ
CSB-EP431650MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L15P (rpl15p) CSB-YP431651MNQ
CSB-EP431651MNQ
CSB-BP431651MNQ
CSB-MP431651MNQ
CSB-EP431651MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S4e (rps4e) CSB-YP431652MNQ
CSB-EP431652MNQ
CSB-BP431652MNQ
CSB-MP431652MNQ
CSB-EP431652MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 5-formaminoimidazole-4-carboxamide-1- (beta)-D-ribofuranosyl 5'-monophosphate synthetase CSB-YP431653MNQ
CSB-EP431653MNQ
CSB-BP431653MNQ
CSB-MP431653MNQ
CSB-EP431653MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribose 1,5-bisphosphate isomerase (MmarC6_1321) CSB-YP431654MNQ
CSB-EP431654MNQ
CSB-BP431654MNQ
CSB-MP431654MNQ
CSB-EP431654MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamyl-tRNA (Gln) amidotransferase subunit D CSB-YP431655MNQ
CSB-EP431655MNQ
CSB-BP431655MNQ
CSB-MP431655MNQ
CSB-EP431655MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable cobalt-precorrin-6Y C (15)-methyltransferase [decarboxylating] CSB-YP431656MNQ
CSB-EP431656MNQ
CSB-BP431656MNQ
CSB-MP431656MNQ
CSB-EP431656MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribose-5-phosphate isomerase A (rpiA) CSB-YP431657MNQ
CSB-EP431657MNQ
CSB-BP431657MNQ
CSB-MP431657MNQ
CSB-EP431657MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Cysteine--tRNA ligase (cysS) CSB-YP431658MNQ
CSB-EP431658MNQ
CSB-BP431658MNQ
CSB-MP431658MNQ
CSB-EP431658MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP431659MNQ
CSB-EP431659MNQ
CSB-BP431659MNQ
CSB-MP431659MNQ
CSB-EP431659MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP431660MNQ
CSB-EP431660MNQ
CSB-BP431660MNQ
CSB-MP431660MNQ
CSB-EP431660MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0254 protein MmarC6_1720 (MmarC6_1720) CSB-YP431661MNQ
CSB-EP431661MNQ
CSB-BP431661MNQ
CSB-MP431661MNQ
CSB-EP431661MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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