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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi B L-rhamnonate dehydratase (rhmD) CSB-YP432243STF
CSB-EP432243STF
CSB-BP432243STF
CSB-MP432243STF
CSB-EP432243STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP432244STF
CSB-EP432244STF
CSB-BP432244STF
CSB-MP432244STF
CSB-EP432244STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0176 protein yceA (yceA) CSB-YP432245STF
CSB-EP432245STF
CSB-BP432245STF
CSB-MP432245STF
CSB-EP432245STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP432246STF
CSB-EP432246STF
CSB-BP432246STF
CSB-MP432246STF
CSB-EP432246STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP432247STF1
CSB-EP432247STF1
CSB-BP432247STF1
CSB-MP432247STF1
CSB-EP432247STF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0352 protein yejL (yejL) CSB-YP432248STF
CSB-EP432248STF
CSB-BP432248STF
CSB-MP432248STF
CSB-EP432248STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP432249STF
CSB-EP432249STF
CSB-BP432249STF
CSB-MP432249STF
CSB-EP432249STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Macrodomain Ter protein (matP) CSB-YP432250STF
CSB-EP432250STF
CSB-BP432250STF
CSB-MP432250STF
CSB-EP432250STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Ribosome-binding factor A (rbfA) CSB-YP432251STF
CSB-EP432251STF
CSB-BP432251STF
CSB-MP432251STF
CSB-EP432251STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B GTPase obg (obg) CSB-YP432252STF
CSB-EP432252STF
CSB-BP432252STF
CSB-MP432252STF
CSB-EP432252STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP432253STF
CSB-EP432253STF
CSB-BP432253STF
CSB-MP432253STF
CSB-EP432253STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Lipoate-protein ligase A (lplA) CSB-YP432254STF
CSB-EP432254STF
CSB-BP432254STF
CSB-MP432254STF
CSB-EP432254STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Deoxycytidine triphosphate deaminase (dcd) CSB-YP432255STF
CSB-EP432255STF
CSB-BP432255STF
CSB-MP432255STF
CSB-EP432255STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Chromosome partition protein mukF (mukF) CSB-YP432256STF
CSB-EP432256STF
CSB-BP432256STF
CSB-MP432256STF
CSB-EP432256STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP432257STF
CSB-EP432257STF
CSB-BP432257STF
CSB-MP432257STF
CSB-EP432257STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP432258STF
CSB-EP432258STF
CSB-BP432258STF
CSB-MP432258STF
CSB-EP432258STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP432259STF
CSB-EP432259STF
CSB-BP432259STF
CSB-MP432259STF
CSB-EP432259STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 50S ribosomal protein L13 (rplM) CSB-YP432260STF
CSB-EP432260STF
CSB-BP432260STF
CSB-MP432260STF
CSB-EP432260STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP432261STF
CSB-EP432261STF
CSB-BP432261STF
CSB-MP432261STF
CSB-EP432261STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Protein grpE (grpE) CSB-YP432262CXH
CSB-EP432262CXH
CSB-BP432262CXH
CSB-MP432262CXH
CSB-EP432262CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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