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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella arizonae UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP431282STE
CSB-EP431282STE
CSB-BP431282STE
CSB-MP431282STE
CSB-EP431282STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribosome maturation factor RimP (rimP) CSB-YP431283STE
CSB-EP431283STE
CSB-BP431283STE
CSB-MP431283STE
CSB-EP431283STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0745 protein YcgL (ycgL) CSB-YP431284STE
CSB-EP431284STE
CSB-BP431284STE
CSB-MP431284STE
CSB-EP431284STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Outer-membrane lipoprotein lolB (lolB) CSB-YP431285STE
CSB-EP431285STE
CSB-BP431285STE
CSB-MP431285STE
CSB-EP431285STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP431286STE
CSB-EP431286STE
CSB-BP431286STE
CSB-MP431286STE
CSB-EP431286STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP431287STE
CSB-EP431287STE
CSB-BP431287STE
CSB-MP431287STE
CSB-EP431287STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP431288STE
CSB-EP431288STE
CSB-BP431288STE
CSB-MP431288STE
CSB-EP431288STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Sugar fermentation stimulation protein A (sfsA) CSB-YP431289STE
CSB-EP431289STE
CSB-BP431289STE
CSB-MP431289STE
CSB-EP431289STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Serine/threonine transporter sstT (sstT), partial CSB-YP431290STE
CSB-EP431290STE
CSB-BP431290STE
CSB-MP431290STE
CSB-EP431290STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae G/U mismatch-specific DNA glycosylase (mug) CSB-YP431291STE
CSB-EP431291STE
CSB-BP431291STE
CSB-MP431291STE
CSB-EP431291STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae GMP reductase (guaC) CSB-YP431292STE
CSB-EP431292STE
CSB-BP431292STE
CSB-MP431292STE
CSB-EP431292STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribulokinase (araB), partial CSB-YP431293STE
CSB-EP431293STE
CSB-BP431293STE
CSB-MP431293STE
CSB-EP431293STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Mannonate dehydratase (uxuA) CSB-YP431294STE
CSB-EP431294STE
CSB-BP431294STE
CSB-MP431294STE
CSB-EP431294STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0235 protein yggU (yggU) CSB-YP431295STE
CSB-EP431295STE
CSB-BP431295STE
CSB-MP431295STE
CSB-EP431295STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Chaperone protein DnaK (dnaK), partial CSB-YP431296STE
CSB-EP431296STE
CSB-BP431296STE
CSB-MP431296STE
CSB-EP431296STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP431297STE
CSB-EP431297STE
CSB-BP431297STE
CSB-MP431297STE
CSB-EP431297STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Chromosome initiation inhibitor (iciA) CSB-YP431298STE
CSB-EP431298STE
CSB-BP431298STE
CSB-MP431298STE
CSB-EP431298STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0267 protein yqfB (yqfB) CSB-YP431299STE
CSB-EP431299STE
CSB-BP431299STE
CSB-MP431299STE
CSB-EP431299STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0482 protein ynfB (ynfB) CSB-YP431300STE
CSB-EP431300STE
CSB-BP431300STE
CSB-MP431300STE
CSB-EP431300STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Electron transport complex protein RnfA (rnfA), partial CSB-YP431301STE1
CSB-EP431301STE1
CSB-BP431301STE1
CSB-MP431301STE1
CSB-EP431301STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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