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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella paratyphi B Acetylglutamate kinase (argB) CSB-YP431342STF
CSB-EP431342STF
CSB-BP431342STF
CSB-MP431342STF
CSB-EP431342STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP431343STF
CSB-EP431343STF
CSB-BP431343STF
CSB-MP431343STF
CSB-EP431343STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP431344STF
CSB-EP431344STF
CSB-BP431344STF
CSB-MP431344STF
CSB-EP431344STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP431345STF
CSB-EP431345STF
CSB-BP431345STF
CSB-MP431345STF
CSB-EP431345STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Ribonuclease HII (rnhB) CSB-YP431346STF
CSB-EP431346STF
CSB-BP431346STF
CSB-MP431346STF
CSB-EP431346STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP431347STF
CSB-EP431347STF
CSB-BP431347STF
CSB-MP431347STF
CSB-EP431347STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B HTH-type transcriptional regulator iscR (iscR) CSB-YP431348STF
CSB-EP431348STF
CSB-BP431348STF
CSB-MP431348STF
CSB-EP431348STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP431349STF
CSB-EP431349STF
CSB-BP431349STF
CSB-MP431349STF
CSB-EP431349STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP431350STF
CSB-EP431350STF
CSB-BP431350STF
CSB-MP431350STF
CSB-EP431350STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Succinylglutamate desuccinylase (astE) CSB-YP431351STF
CSB-EP431351STF
CSB-BP431351STF
CSB-MP431351STF
CSB-EP431351STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP431352STF
CSB-EP431352STF
CSB-BP431352STF
CSB-MP431352STF
CSB-EP431352STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B L-fucose isomerase (fucI), partial CSB-YP431353STF
CSB-EP431353STF
CSB-BP431353STF
CSB-MP431353STF
CSB-EP431353STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0370 protein ypfN (ypfN), partial CSB-YP431354STF
CSB-EP431354STF
CSB-BP431354STF
CSB-MP431354STF
CSB-EP431354STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP431355STF
CSB-EP431355STF
CSB-BP431355STF
CSB-MP431355STF
CSB-EP431355STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0267 protein yqfB (yqfB) CSB-YP431356STF
CSB-EP431356STF
CSB-BP431356STF
CSB-MP431356STF
CSB-EP431356STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Arginine exporter protein ArgO (argO), partial CSB-YP431357STF1
CSB-EP431357STF1
CSB-BP431357STF1
CSB-MP431357STF1
CSB-EP431357STF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 60 kDa chaperonin (groL), partial CSB-YP431358STF
CSB-EP431358STF
CSB-BP431358STF
CSB-MP431358STF
CSB-EP431358STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP431359STF
CSB-EP431359STF
CSB-BP431359STF
CSB-MP431359STF
CSB-EP431359STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP431360STF
CSB-EP431360STF
CSB-BP431360STF
CSB-MP431360STF
CSB-EP431360STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B High frequency lysogenization protein HflD homolog (hflD) CSB-YP431361STF
CSB-EP431361STF
CSB-BP431361STF
CSB-MP431361STF
CSB-EP431361STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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