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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Gluconacetobacter diazotrophicus Ribonuclease P protein component (rnpA) CSB-YP433603GEN
CSB-EP433603GEN
CSB-BP433603GEN
CSB-MP433603GEN
CSB-EP433603GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Adenylosuccinate synthetase (purA) CSB-YP433604GEN
CSB-EP433604GEN
CSB-BP433604GEN
CSB-MP433604GEN
CSB-EP433604GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 30S ribosomal protein S2 (rpsB) CSB-YP433605GEN
CSB-EP433605GEN
CSB-BP433605GEN
CSB-MP433605GEN
CSB-EP433605GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 30S ribosomal protein S21 (rpsU) CSB-YP433606GEN
CSB-EP433606GEN
CSB-BP433606GEN
CSB-MP433606GEN
CSB-EP433606GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 8-amino-7-oxononanoate synthase (bioF) CSB-YP433607BPB
CSB-EP433607BPB
CSB-BP433607BPB
CSB-MP433607BPB
CSB-EP433607BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP433608BPB
CSB-EP433608BPB
CSB-BP433608BPB
CSB-MP433608BPB
CSB-EP433608BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Leucine--tRNA ligase (leuS), partial CSB-YP433609BPB
CSB-EP433609BPB
CSB-BP433609BPB
CSB-MP433609BPB
CSB-EP433609BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP433610BPB
CSB-EP433610BPB
CSB-BP433610BPB
CSB-MP433610BPB
CSB-EP433610BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Uracil phosphoribosyltransferase (upp) CSB-YP433611BPB
CSB-EP433611BPB
CSB-BP433611BPB
CSB-MP433611BPB
CSB-EP433611BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Homoserine O-acetyltransferase (metX) CSB-YP433612BPB
CSB-EP433612BPB
CSB-BP433612BPB
CSB-MP433612BPB
CSB-EP433612BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii HPr kinase/phosphorylase (hprK) CSB-YP433613BPB
CSB-EP433613BPB
CSB-BP433613BPB
CSB-MP433613BPB
CSB-EP433613BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP433614BPB
CSB-EP433614BPB
CSB-BP433614BPB
CSB-MP433614BPB
CSB-EP433614BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii FMN-dependent NADH-azoreductase (azoR) CSB-YP433615BPB
CSB-EP433615BPB
CSB-BP433615BPB
CSB-MP433615BPB
CSB-EP433615BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP433616BPB
CSB-EP433616BPB
CSB-BP433616BPB
CSB-MP433616BPB
CSB-EP433616BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L25 (rplY) CSB-YP433617BPB
CSB-EP433617BPB
CSB-BP433617BPB
CSB-MP433617BPB
CSB-EP433617BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP433618BPB
CSB-EP433618BPB
CSB-BP433618BPB
CSB-MP433618BPB
CSB-EP433618BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L21 (rplU) CSB-YP433619BPB
CSB-EP433619BPB
CSB-BP433619BPB
CSB-MP433619BPB
CSB-EP433619BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP433620BPB
CSB-EP433620BPB
CSB-BP433620BPB
CSB-MP433620BPB
CSB-EP433620BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 30S ribosomal protein S15 (rpsO) CSB-YP433621BPB
CSB-EP433621BPB
CSB-BP433621BPB
CSB-MP433621BPB
CSB-EP433621BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Type III pantothenate kinase (coaX) CSB-YP433622BPB
CSB-EP433622BPB
CSB-BP433622BPB
CSB-MP433622BPB
CSB-EP433622BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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