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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bordetella petrii UPF0434 protein Bpet2671 (Bpet2671) CSB-YP433643BPB
CSB-EP433643BPB
CSB-BP433643BPB
CSB-MP433643BPB
CSB-EP433643BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Dihydroorotate dehydrogenase (quinone) CSB-YP433644BOS
CSB-EP433644BOS
CSB-BP433644BOS
CSB-MP433644BOS
CSB-EP433644BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum ATP synthase subunit b 1 (atpF1), partial CSB-YP433645BOS1
CSB-EP433645BOS1
CSB-BP433645BOS1
CSB-MP433645BOS1
CSB-EP433645BOS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Co-chaperone protein hscB homolog (hscB) CSB-YP433646BPB
CSB-EP433646BPB
CSB-BP433646BPB
CSB-MP433646BPB
CSB-EP433646BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Peptidyl-tRNA hydrolase (pth) CSB-YP433647BOS
CSB-EP433647BOS
CSB-BP433647BOS
CSB-MP433647BOS
CSB-EP433647BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP433648BPB
CSB-EP433648BPB
CSB-BP433648BPB
CSB-MP433648BPB
CSB-EP433648BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum DNA repair protein recO (recO) CSB-YP433649BOS
CSB-EP433649BOS
CSB-BP433649BOS
CSB-MP433649BOS
CSB-EP433649BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 50S ribosomal protein L9 (rplI) CSB-YP433650BOS
CSB-EP433650BOS
CSB-BP433650BOS
CSB-MP433650BOS
CSB-EP433650BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP433651BOS
CSB-EP433651BOS
CSB-BP433651BOS
CSB-MP433651BOS
CSB-EP433651BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 50S ribosomal protein L7/L12 (rplL) CSB-YP433652BOS
CSB-EP433652BOS
CSB-BP433652BOS
CSB-MP433652BOS
CSB-EP433652BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Glutamate--tRNA ligase 1 (gltX1) CSB-YP433653BOS
CSB-EP433653BOS
CSB-BP433653BOS
CSB-MP433653BOS
CSB-EP433653BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L9 (rplI) CSB-YP433654BPB
CSB-EP433654BPB
CSB-BP433654BPB
CSB-MP433654BPB
CSB-EP433654BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP433655BPB
CSB-EP433655BPB
CSB-BP433655BPB
CSB-MP433655BPB
CSB-EP433655BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Aspartate carbamoyltransferase (pyrB) CSB-YP433656BOS
CSB-EP433656BOS
CSB-BP433656BOS
CSB-MP433656BOS
CSB-EP433656BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP433657BOS
CSB-EP433657BOS
CSB-BP433657BOS
CSB-MP433657BOS
CSB-EP433657BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP433658BOS
CSB-EP433658BOS
CSB-BP433658BOS
CSB-MP433658BOS
CSB-EP433658BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP433659BOS
CSB-EP433659BOS
CSB-BP433659BOS
CSB-MP433659BOS
CSB-EP433659BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Aspartate--tRNA ligase (aspS), partial CSB-YP433660BOS
CSB-EP433660BOS
CSB-BP433660BOS
CSB-MP433660BOS
CSB-EP433660BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 30S ribosomal protein S9 (rpsI) CSB-YP433661BOS
CSB-EP433661BOS
CSB-BP433661BOS
CSB-MP433661BOS
CSB-EP433661BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 50S ribosomal protein L15 (rplO) CSB-YP433662BOS
CSB-EP433662BOS
CSB-BP433662BOS
CSB-MP433662BOS
CSB-EP433662BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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