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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella arizonae Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP433043STE
CSB-EP433043STE
CSB-BP433043STE
CSB-MP433043STE
CSB-EP433043STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Pantothenate synthetase (panC) CSB-YP433044STE
CSB-EP433044STE
CSB-BP433044STE
CSB-MP433044STE
CSB-EP433044STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae D-tagatose-1,6-bisphosphate aldolase subunit kbaZ (kbaZ) CSB-YP433045STE
CSB-EP433045STE
CSB-BP433045STE
CSB-MP433045STE
CSB-EP433045STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Multifunctional CCA protein (cca) CSB-YP433046STE
CSB-EP433046STE
CSB-BP433046STE
CSB-MP433046STE
CSB-EP433046STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Zinc transporter ZupT (zupT), partial CSB-YP433047STE1
CSB-EP433047STE1
CSB-BP433047STE1
CSB-MP433047STE1
CSB-EP433047STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP433048STE
CSB-EP433048STE
CSB-BP433048STE
CSB-MP433048STE
CSB-EP433048STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae L-arabinose isomerase (araA) CSB-YP433049STE
CSB-EP433049STE
CSB-BP433049STE
CSB-MP433049STE
CSB-EP433049STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP433050STE
CSB-EP433050STE
CSB-BP433050STE
CSB-MP433050STE
CSB-EP433050STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP433051STE
CSB-EP433051STE
CSB-BP433051STE
CSB-MP433051STE
CSB-EP433051STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Carnitinyl-CoA dehydratase (caiD) CSB-YP433052STE
CSB-EP433052STE
CSB-BP433052STE
CSB-MP433052STE
CSB-EP433052STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 30S ribosomal protein S20 (rpsT) CSB-YP433053STE
CSB-EP433053STE
CSB-BP433053STE
CSB-MP433053STE
CSB-EP433053STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Non-canonical purine NTP phosphatase (yjjX) CSB-YP433054STE
CSB-EP433054STE
CSB-BP433054STE
CSB-MP433054STE
CSB-EP433054STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP433055STE
CSB-EP433055STE
CSB-BP433055STE
CSB-MP433055STE
CSB-EP433055STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Transcriptional repressor rcnR (rcnR) CSB-YP433056STE
CSB-EP433056STE
CSB-BP433056STE
CSB-MP433056STE
CSB-EP433056STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Probable sugar efflux transporter (sotB), partial CSB-YP433057STE
CSB-EP433057STE
CSB-BP433057STE
CSB-MP433057STE
CSB-EP433057STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Electron transport complex protein RnfC (rnfC), partial CSB-YP433058STE
CSB-EP433058STE
CSB-BP433058STE
CSB-MP433058STE
CSB-EP433058STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae L-fucose isomerase (fucI), partial CSB-YP433059STE
CSB-EP433059STE
CSB-BP433059STE
CSB-MP433059STE
CSB-EP433059STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0194 membrane protein ybhG (ybhG) CSB-YP433060STF
CSB-EP433060STF
CSB-BP433060STF
CSB-MP433060STF
CSB-EP433060STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 50S ribosomal protein L5 (rplE) CSB-YP433061STF
CSB-EP433061STF
CSB-BP433061STF
CSB-MP433061STF
CSB-EP433061STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 50S ribosomal protein L2 (rplB) CSB-YP433062STF
CSB-EP433062STF
CSB-BP433062STF
CSB-MP433062STF
CSB-EP433062STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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